Collagens are the most abundant proteins in vertebrates and constitute the major components of the extracellular matrix. Collagens play an important and multifaceted role in the development and functioning of the nervous system and undergo structural remodeling and quantitative modifications during aging. Here, we investigated the age-dependent regulation of and in the brain of the short-lived vertebrate , a powerful model organism for aging research due to its natural fast-aging process and further characterized typical hallmarks of brain aging in this species. We showed that and are relatively well conserved during vertebrate evolution, and their expression significantly increases in the brain of upon aging. Noteworthy, we report that both and are expressed in cells with a neuronal phenotype, unlike what has already been documented in mammalian brain, in which only is considered a neuronal marker, whereas seems to be expressed only in endothelial cells. Overall, our findings encourage further investigation on the role of and in the biology of the vertebrate brain as well as the onset of aging and neurodegenerative diseases.