Vuk-Pavlović S, Opara E C, Levanat S, Vrbanec D, Pavelić K
Cancer Res. 1986 May;46(5):2208-13.
A substance immunochemically cross-reactive with insulin (SICRI) appears in melanoma B16 growing in diabetic and nondiabetic C57BL/6 mice. Progression of tumor size is paralleled by the increase of SICRI levels in the serum of both diabetic and nondiabetic animals; this increase correlates with a decreased concentration of circulating glucose and an elevated concentration of growth hormone in blood. Melanoma B16 grown under serum-free culture conditions secretes SICRI into the medium. Affinity-purified SICRI stimulates glucose uptake by rat epididymal adipocytes and competes with radiolabeled insulin for binding to these cells. Low concentrations of SICRI enhance growth of cultured melanoma B16 cells, whereas high concentrations of this substance have inhibitory growth effects on these cells. Porcine insulin, human insulin-like growth factors I and II, human growth hormone, platelet-derived growth factor, epidermal growth factor, and fibroblast growth factor have negligible influence on growth of melanoma B16.
在糖尿病和非糖尿病的C57BL/6小鼠体内生长的黑色素瘤B16中，出现了一种与胰岛素免疫化学交叉反应的物质（SICRI）。在糖尿病和非糖尿病动物的血清中，肿瘤大小的进展与SICRI水平的升高平行；这种升高与循环葡萄糖浓度降低和血液中生长激素浓度升高相关。在无血清培养条件下生长的黑色素瘤B16将SICRI分泌到培养基中。亲和纯化的SICRI刺激大鼠附睾脂肪细胞摄取葡萄糖，并与放射性标记的胰岛素竞争结合这些细胞。低浓度的SICRI促进培养的黑色素瘤B16细胞生长，而高浓度的该物质对这些细胞具有生长抑制作用。猪胰岛素、人胰岛素样生长因子I和II、人生长激素、血小板衍生生长因子、表皮生长因子和成纤维细胞生长因子对黑色素瘤B16的生长影响可忽略不计。
