Department of Chemistry, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Aljouf, Saudi Arabia.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy St., Beni-Suef 62574, Egypt.
Molecules. 2022 Jan 24;27(3):764. doi: 10.3390/molecules27030764.
Combination therapy of many anthelmintic drugs has been used to achieve fast animal curing. Q-DRENCH is an oral suspension, containing four different active drugs against GIT worms in sheep, commonly used in Australia and New Zeeland. The anti-parasitic drugs are Albendazole (ALB), Levamisole HCl (LEV), Abamectin (ABA), and Closantel (CLO). The main purpose of this study is to present a new simultaneous stability-indicting HPLC-DAD method for the analysis of the four drugs. The recommended liquid system was 1 mL of Triethylamine/L water, adjusting the pH to 3.5 by glacial acetic acid: acetonitrile solvent (20:80, /). Isocratic elusion achieved the desired results of separation at a 2 mL/min flow rate using Zorbax C-18 as a stationary phase. Detection was performed at 210 nm. The linearity ranges were 15.15 to 93.75 μg/mL for ALB, 25 to 150 μg/mL for LEV, 30 to 150 μg/mL for ABA, and 11.7 to 140.63 μg/mL for CLO. Moreover, the final greenness score was 0.62 using the AGREE tool, which reflects the eco-friendly nature. Moreover, the four drugs were determined successfully in the presence of their stressful degradation products. This work presents the first chromatographic method for simultaneous analysis for Q-DRENCH oral suspension drugs in the presence of their stressful degradation products.
联合使用多种驱虫药物进行治疗，以实现快速治愈动物。Q-DRENCH 是一种口服混悬液，含有四种不同的抗绵羊胃肠道蠕虫的活性药物，在澳大利亚和新西兰广泛使用。这些抗寄生虫药物分别是阿苯达唑（ALB）、左旋咪唑盐酸盐（LEV）、阿维菌素（ABA）和氯氰碘柳胺（CLO）。本研究的主要目的是介绍一种新的同时指示稳定性的 HPLC-DAD 方法，用于分析这四种药物。推荐的液相系统为 1 毫升三乙胺/L 水，用冰醋酸调节 pH 值至 3.5：乙腈溶剂（20:80，/）。等度洗脱在 2 毫升/分钟的流速下使用 Zorbax C-18 作为固定相，实现了所需的分离效果。检测波长为 210nm。ALB 的线性范围为 15.15 至 93.75μg/mL，LEV 的线性范围为 25 至 150μg/mL，ABA 的线性范围为 30 至 150μg/mL，CLO 的线性范围为 11.7 至 140.63μg/mL。此外，使用 AGREE 工具评估的最终绿色评分得分为 0.62，反映了该方法的环保特性。此外，该方法还成功地在存在应激降解产物的情况下测定了这四种药物。本工作首次报道了一种在存在应激降解产物的情况下，同时分析 Q-DRENCH 口服混悬液中药物的色谱方法。
