Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah 22254, Saudi Arabia.
Biology Department, Faculty of Science, King Abdulaziz University, Jeddah 22254, Saudi Arabia.
Molecules. 2022 Feb 2;27(3):1027. doi: 10.3390/molecules27031027.
Diabetes mellitus is a common global health problem. Among the complications that are frequently associated with DM are the alternation of sexual function and fertility, especially in young men. This study aimed to assess the efficacy of nanoparticles of () in preserving the prostatic structure of diabetic rats and to explore the mechanism behind this effect. A model of DM was induced in male albino rats by a single intraperitoneally injection of streptozotocin (STZ, 60 mg/kg body weight). Five groups ( = 10 each) of rats were included in this study: the control, gold nanoparticles-treated (150 mg/kg body weight through gastric intubation for 30 days), untreated diabetic, metformin-treated diabetic (500 mg/kg/day gastric intubation for 30 days) and the -treated diabetic group. The blood glucose, insulin and testosterone levels as well as oxidants/antioxidants status were assessed in the serum. Gene expression of proinflammatory cytokines TNF-α, IL1β and IL-6 were assessed in the prostate homogenate. At the end of the experiment, the rats were sacrificed and the prostate was dissected out and prepared for histopathological and immunohistochemistry study using Ki67 and Bcl-2. nanoparticles significantly decreased ( = 0.03) the blood glucose level while significantly increasing insulin ( = 0.01) and testosterone ( = 0.04) levels compared to the untreated diabetic rats. Oxidants/antioxidants status was markedly improved after administration of Prostatic expression of the mRNA of pro-inflammatory cytokines IL-6, IL1β and TNF-α was down-regulated in metformin- and -treated rats. The histological structure of the ventral prostate was preserved in metformin- and -treated diabetic rats with a significantly thicker epithelial cell layer and significant increase immunoexpression in Bcl-2 and Ki67. In conclusion, the protective effect induced by nanoparticles on the prostate of diabetic rats might be directly mediated through the down-regulation of inflammatory cytokines and the up-regulation of antioxidant activity and indirectly mediated through the anti-hyperglycemic effect through enhancing insulin secretion.
糖尿病是一种常见的全球性健康问题。糖尿病常伴有性功能和生育能力改变等并发症，尤其是在年轻男性中。本研究旨在评估 () 纳米粒子对糖尿病大鼠前列腺结构的保护作用，并探讨其作用机制。通过单次腹腔注射链脲佐菌素（STZ，60mg/kg 体重）诱导雄性白化大鼠糖尿病模型。本研究纳入了 5 组大鼠（每组 10 只）：对照组、金纳米粒子治疗组（150mg/kg 体重，通过胃管灌胃 30 天）、未治疗糖尿病组、二甲双胍治疗糖尿病组（500mg/kg/天，胃管灌胃 30 天）和 - 治疗糖尿病组。检测血清中血糖、胰岛素和睾酮水平以及氧化应激/抗氧化状态。评估前列腺匀浆中促炎细胞因子 TNF-α、IL1β 和 IL-6 的基因表达。实验结束时，处死大鼠，取出前列腺，进行组织病理学和 Ki67 和 Bcl-2 免疫组织化学研究。与未治疗糖尿病大鼠相比，金纳米粒子治疗组的血糖水平显著降低（ = 0.03），胰岛素（ = 0.01）和睾酮（ = 0.04）水平显著升高。氧化应激/抗氧化状态在给予金纳米粒子治疗后明显改善。与二甲双胍和 - 治疗大鼠相比，促炎细胞因子 IL-6、IL1β 和 TNF-α 的 mRNA 在前列腺中的表达下调。在二甲双胍和 - 治疗的糖尿病大鼠中，腹侧前列腺的组织学结构得到了保存，上皮细胞层明显增厚，Bcl-2 和 Ki67 的免疫表达显著增加。总之，金纳米粒子对糖尿病大鼠前列腺的保护作用可能是通过下调炎症细胞因子和上调抗氧化活性直接介导的，通过增强胰岛素分泌的抗高血糖作用间接介导的。
