某些植物提取物对链脲佐菌素诱导的雄性白化病大鼠 2 型糖尿病的降血糖作用。
The Anti-Diabetic Effect of Some Plant Extracts Against Streptozotocin - Induced Diabetes Type 2 in Male Albino Rats.
机构信息
Zoology Department, Faculty of Science, Ain-Sham University, Cairo, Egypt.
Center of biotechnology of Sfax, Biology Sciences Department, College of Sciences & Arts-Scientific Departments, Qassim University, Buraydah, Saudi Arabia.
出版信息
Endocr Metab Immune Disord Drug Targets. 2021;21(8):1431-1440. doi: 10.2174/1871530320666201016145502.
BACKGROUND
One of the widely spread disorders is Diabetes mellitus, especially type 2 (T2DM). T2DM is attributed to the change in life style and stress. A possible strategy to block dietary carbohydrate absorption is regulation of postprandial blood glucose level as well, the use of some natural plant extracts with inhibitory effect against carbohydrate digestive enzymes such as alpha- amylase and fewer side effects than synthetic drugs. This study was conducted to investigate the anti-diabetic effect of Cinnamon and Saussurea extract, individually, on blood glucose, lipid profile, insulin, interleukin1-beta and weight loss in diabetic rats treated with Streptozotocin (STZ).
METHODS
The experiment was performed on 60 Wistar male rats; the experimental study include 6 groups (10 rats each): (I) normal rats, (II) Streptozotocin- induced diabetic rats, (III) normal rats orally received (200 mg/kg/day) Saussurea ethanolic extract (SEE) for consecutive 4 weeks, (IV) normal rats orally received (100mg/kg/day) Cinnamon aqueous extract (CAE) for consecutive 4 weeks, (V) Streptozotocin -treated rats received SEE orally (200mg /kg/ day) for consecutive 4 weeks, and (VI) Streptozotocin -treated rats received CAE orally (100mg /kg/ day) for consecutive 4 weeks.
RESULTS
The results of the following study revealed that SEE has more anti-diabetic effect against Streptozotocin treatment than CAE due to the high α-amylase inhibition potential and higher phenolic content. Also, GC-MS analysis of SEE exhibited higher concentrations of phenolic compounds such as: dehydrocostus lactone, azuleno, eicosa-pentaenoic acid and linoelaidic acid that revealed anti-diabetic, anti-lipidemic and anti-inflammatory activities, while CAE showed the presence of cinnamic and quinic acids. Injection of STZ resulted in a decline in the insulin, high density lipoprotein and body weight values matched with the increase in glucose, total cholesterol, LDL-Cholesterol, triglycerides and interleukin1- β (IL-1β). The administration of extracts of SEE and CAE into STZ-treated rats separately resulted in a decline in the elevated levels of blood glucose, total cholesterol, triglycerides and improving serum HDL-Cholesterol and body weight.
CONCLUSION
Both tested herbal extracts performed anti-diabetic effect that mainly could be mechanized via the α-amylase- inhibitory potentials due to the high phenolic and flavonoids content.
背景
糖尿病是一种广泛传播的疾病，尤其是 2 型糖尿病（T2DM）。T2DM 归因于生活方式和压力的改变。一种可能的策略是通过调节餐后血糖水平来阻止膳食碳水化合物的吸收，使用一些具有抑制碳水化合物消化酶（如α-淀粉酶）作用的天然植物提取物，其副作用比合成药物少。本研究旨在研究肉桂和雪莲提取物单独对链脲佐菌素（STZ）治疗的糖尿病大鼠的血糖、血脂谱、胰岛素、白细胞介素 1-β和体重减轻的抗糖尿病作用。
方法
本实验在 60 只 Wistar 雄性大鼠上进行；实验研究包括 6 组（每组 10 只）：（I）正常大鼠，（II）链脲佐菌素诱导的糖尿病大鼠，（III）正常大鼠连续 4 周口服（200mg/kg/天）雪莲乙醇提取物（SEE），（IV）正常大鼠连续 4 周口服（100mg/kg/天）肉桂水提取物（CAE），（V）链脲佐菌素治疗大鼠连续 4 周口服 SEE（200mg/kg/天），（VI）链脲佐菌素治疗大鼠连续 4 周口服 CAE（100mg/kg/天）。
结果
研究结果表明，由于高α-淀粉酶抑制潜力和高酚含量，SEE 对链脲佐菌素治疗的抗糖尿病作用强于 CAE。此外，SEE 的 GC-MS 分析显示出较高浓度的酚类化合物，如脱氢木香内酯、蓝烯、二十碳五烯酸和亚油酸，具有抗糖尿病、抗脂和抗炎活性，而 CAE 则显示出肉桂酸和奎宁酸的存在。STZ 注射导致胰岛素、高密度脂蛋白和体重值下降，同时葡萄糖、总胆固醇、LDL-胆固醇、甘油三酯和白细胞介素 1-β（IL-1β）升高。分别将 SEE 和 CAE 提取物给予 STZ 治疗的大鼠，可降低血糖、总胆固醇、甘油三酯水平，并改善血清 HDL-胆固醇和体重。
结论
两种经测试的草药提取物均具有抗糖尿病作用，主要可通过α-淀粉酶抑制潜力来实现，这主要归因于高酚类和类黄酮含量。
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