涡流液介导的催产素的亚硫酸化氧化脱硫醇反应。
Vortex Fluidic Mediated Oxidative Sulfitolysis of Oxytocin.
机构信息
Flinders Institute for Nanoscale Science and Technology, College of Science and Engineering, Flinders University, Adelaide, SA 5042, Australia.
Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia.
出版信息
Molecules. 2022 Feb 7;27(3):1109. doi: 10.3390/molecules27031109.
In peptide production, oxidative sulfitolysis can be used to protect the cysteine residues during purification, and the introduction of a negative charge aids solubility. Subsequent controlled reduction aids in ensuring correct disulfide bridging. In vivo, these problems are overcome through interaction with chaperones. Here, a versatile peptide production process has been developed using an angled vortex fluidic device (VFD), which expands the viable pH range of oxidative sulfitolysis from pH 10.5 under batch conditions, to full conversion within 20 min at pH 9-10.5 utilising the VFD. VFD processing gave 10-fold greater conversion than using traditional batch processing, which has potential in many applications of the sulfitolysis reaction.
在肽生产中，氧化亚硫酸酯裂解可用于在纯化过程中保护半胱氨酸残基，并且引入负电荷有助于提高溶解度。随后的受控还原有助于确保正确的二硫键桥接。在体内，这些问题通过与伴侣蛋白相互作用得到解决。在这里，使用倾斜式涡旋流控装置（VFD）开发了一种通用的肽生产工艺，该工艺将氧化亚硫酸酯裂解在分批条件下的可行 pH 范围从 10.5 扩展到 pH 9-10.5 下 20 分钟内完全转化。VFD 处理比传统的分批处理转化率高 10 倍，这在亚硫酸酯裂解反应的许多应用中具有潜力。
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