State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
Anal Bioanal Chem. 2022 Jul;414(18):5121-5138. doi: 10.1007/s00216-022-03945-4. Epub 2022 Feb 14.
Immunoassay is one of the most important clinical techniques for disease/pathological diagnosis. Mass spectrometry (MS) has been a popular and powerful readout technique for immunoassays, generating the mass spectrometric immunoassays (MSIAs) with unbeatable channels for multiplexed detection. The sensitivity of MSIAs has been greatly improved with the development of mass labels from element labels to small-molecular labels. MSIAs are also expended from the representative element MS-based methods to the laser-based organic MS and latest ambient MS, improving in both technology and methodology. Various MSIAs present high potential for clinical applications, including the biomarker screening, the immunohistochemistry, and the advanced single-cell analysis. Here, we give an overall review of the development of MSIAs in recent years, highlighting the latest improvement of mass labels and MS techniques for clinical immunoassays.
免疫分析是疾病/病理诊断的最重要的临床技术之一。质谱（MS）一直是免疫分析的一种流行且强大的读出技术，产生了具有无与伦比的多重检测通道的质谱免疫分析（MSIA）。随着元素标记物向小分子标记物的发展，MSIA 的灵敏度得到了极大提高。MSIA 也从代表性的基于元素 MS 的方法扩展到基于激光的有机 MS 和最新的环境 MS，在技术和方法学方面都得到了改进。各种 MSIA 具有很高的临床应用潜力，包括生物标志物筛选、免疫组织化学和先进的单细胞分析。在这里，我们对近年来 MSIA 的发展进行了全面综述，重点介绍了用于临床免疫分析的质量标记物和 MS 技术的最新改进。
