Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657 Japan.
Department of Genomics and Evolutionary Biology, National Institute of Genetics, Mishima, Shizuoka, 411-8540 Japan.
Plant Cell Physiol. 2022 Feb 15;63(2):265-278. doi: 10.1093/pcp/pcab169.
The molecular basis for leaf development, a major focus in developmental biology, remains unclear in the monocotyledonous grass, rice (Oryza sativa). Here, we performed a mutant screen in rice and identified an AP2-type transcription factor family protein, NARROW AND DWARF LEAF1 (NDL1). NDL1 is the ortholog of Arabidopsis thaliana (subsequently called Arabidopsis) ENHANCER OF SHOOT REGENERATION1 (ESR1)/DORNRÖSCHEN (DRN) and mediates leaf development and maintenance of the shoot apical meristem (SAM). Loss of function of NDL1 results in bladeless leaves and SAMs that are flat, rather than dome-shaped, and lack cell proliferation activity. This loss of function also causes reduced auxin signaling. Moreover, as is the case with Arabidopsis ESR1/DRN, NDL1 plays crucial roles in shoot regeneration. Importantly, we found that NDL1 is not expressed in the SAM but is expressed in leaf primordia. We propose that NDL1 cell autonomously regulates leaf development, but non-cell autonomously regulates SAM maintenance in rice.
单子叶植物水稻（Oryza sativa）中叶片发育的分子基础是发育生物学的主要焦点，但仍不清楚。在这里，我们在水稻中进行了突变体筛选，鉴定出一个 AP2 型转录因子家族蛋白，窄叶和矮化叶 1（NDL1）。NDL1 是拟南芥（随后称为拟南芥）增强再生能力的 SHOOT1（ESR1）/DRN 的同源物，并介导叶片发育和茎顶端分生组织（SAM）的维持。NDL1 的功能丧失导致无叶和 SAM 呈扁平状，而不是穹顶状，并且缺乏细胞增殖活性。这种功能丧失还导致生长素信号转导减少。此外，与拟南芥 ESR1/DRN 一样，NDL1 在芽再生中也起着至关重要的作用。重要的是，我们发现 NDL1 不在 SAM 中表达，而是在叶原基中表达。我们提出 NDL1 细胞自主调节叶片发育，但非细胞自主调节水稻 SAM 的维持。
