Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, PR China.
Food Funct. 2022 Mar 7;13(5):2594-2605. doi: 10.1039/d1fo02482e.
It is of great significance to develop a dietary intervention strategy to prevent inflammatory bowel disease (IBD). A millet-rich diet can ameliorate IBD, but the active ingredients and mechanisms remain to be studied. Our results showed that the oral administration of foxtail millet protein hydrolysates (FMPH) reduced the disease activity index (DAI) score and improved the colon symptoms of dextran sulfate sodium (DSS)-induced colitis mice. FMPH reduced the serum LPS level, increased intestinal ZO-1 and occludin expression, inhibited NF-κB phosphorylation, and reduced the levels of TNF-α and IL-6. Further, FMPH inhibited Th17 cell differentiation, and inhibited inflammasome activation and IL-1β expression through the NLRP3/ASC/caspase-1 pathway. The results on Caco-2 cells confirmed the role of FMPH on tight junction and inflammasomes activation. A total of 2620 peptides were identified in FMPH by UPLC-MS/MS, of which 22 peptides were predicted as potential biopeptides, and the key sequences were LPF, ANP, PY, YW, and IPP. This study supports the effect of a diet rich in millet on the improvement of IBD and provides a scientific basis for the use of millet protein as a functional food to improve intestinal inflammation.
开发饮食干预策略来预防炎症性肠病（IBD）具有重要意义。富含小米的饮食可以改善 IBD，但活性成分和机制仍有待研究。我们的结果表明，口服黍蛋白水解物（FMPH）可降低葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠的疾病活动指数（DAI）评分并改善结肠症状。FMPH 降低了血清 LPS 水平，增加了肠道 ZO-1 和闭合蛋白的表达，抑制了 NF-κB 磷酸化，并降低了 TNF-α和 IL-6 的水平。此外，FMPH 通过 NLRP3/ASC/caspase-1 途径抑制了 Th17 细胞分化，并抑制了炎症小体的激活和 IL-1β 的表达。在 Caco-2 细胞上的结果证实了 FMPH 对紧密连接和炎症小体激活的作用。通过 UPLC-MS/MS 在 FMPH 中鉴定出 2620 种肽，其中 22 种肽被预测为潜在的生物活性肽，关键序列为 LPF、ANP、PY、YW 和 IPP。本研究支持富含小米的饮食对改善 IBD 的作用，并为将小米蛋白作为改善肠道炎症的功能性食品提供了科学依据。
