Li Cheng, Zhai Shirui, Duan Mengyao, Cao Li, Zhang Jie, Wang Yao, Wu Ying, Gu Shaobin
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China.
National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471023, China.
Nutrients. 2024 Nov 29;16(23):4142. doi: 10.3390/nu16234142.
Probiotics have great potential in improving acute alcohol intoxication. The aim of this study was to investigate the mitigating effect and mechanism of action of BC99 on acute alcohol intoxication (AAI) in SD rats. BC99 was divided into different doses administered by gavage to rats, and a rat model of acute alcohol intoxication was established by multiple gavages of excess alcohol. Our study demonstrated that BC99 intervention significantly prolonged the latency period of intoxication; significantly attenuated alcohol-induced lipid elevation, liver injury, hepatic inflammation, and intestinal barrier damage; and lowered plasma endotoxin (LPS) levels in rats. In addition, BC99 could effectively restore the balance of intestinal flora, increase the abundance of _, _, , and , and thus increase the content of intestinal short-chain fatty acids (SCFAs), especially butyric acid. Moreover, we demonstrated through sodium butyrate validation experiments that butyrate could attenuate intestinal barrier damage and reduce the diffusion of LPS, thereby reducing liver inflammation. In conclusion, BC99 ameliorates acute alcohol intoxication in rats by increasing the abundance of butyrate-producing genera and thereby increasing butyrate abundance to alleviate intestinal barrier injury.
益生菌在改善急性酒精中毒方面具有巨大潜力。本研究旨在探讨BC99对SD大鼠急性酒精中毒（AAI）的缓解作用及作用机制。将BC99分为不同剂量经口灌胃给予大鼠，并通过多次灌胃过量酒精建立大鼠急性酒精中毒模型。我们的研究表明，BC99干预显著延长了中毒潜伏期；显著减轻了酒精诱导的血脂升高、肝损伤、肝脏炎症和肠屏障损伤；并降低了大鼠血浆内毒素（LPS）水平。此外，BC99可有效恢复肠道菌群平衡，增加_、_、_和_的丰度，从而增加肠道短链脂肪酸（SCFAs）的含量，尤其是丁酸。此外，我们通过丁酸钠验证实验证明，丁酸盐可减轻肠屏障损伤并减少LPS的扩散，从而减轻肝脏炎症。总之，BC99通过增加产丁酸菌属的丰度，从而增加丁酸盐丰度以减轻肠屏障损伤，改善大鼠急性酒精中毒。
