Spitsbergen J M, Schat K A
Dev Comp Immunol. 1986 Winter;10(1):11-23. doi: 10.1016/0145-305x(86)90040-6.
This report describes the development of a microtiter system for measuring spontaneous hemolytic activity and immune hemolysins in rainbow trout serum. The chromium release assay, utilizing complement-mediated lysis of heterologous erythrocytes allows rapid, efficient quantitation of hemolytic activity in small serum samples from numerous fish.
本报告描述了一种微量滴定系统的开发,该系统用于测量虹鳟鱼血清中的自发溶血活性和免疫溶血素。铬释放试验利用补体介导的异源红细胞裂解,能够快速、高效地定量来自大量鱼类的小血清样本中的溶血活性。