鲑鱼红细胞内过氧化氢酶和超氧化物歧化酶活性的比较研究。

Comparative studies of catalase and superoxide dismutase activity within salmon fish erythrocytes.

Scott E M, Harrington J P

Department of Chemistry, University of Alaska, Anchorage 99508.

Comp Biochem Physiol B. 1990;95(1):91-3. doi: 10.1016/0305-0491(90)90253-p.

Abstract

Superoxide dismutase isolated from erythrocytes of several species of salmon and the rainbow trout exhibited single electrophoretic bands of activity which migrated anodally similar to the human erythrocyte enzyme; two discrete bands were observed for the coho salmon. 2. No polymorphism was observed for 30 samples from sockeye salmon and six samples from king salmon. Only one sample of rainbow trout (one of 12) exhibited an electrophoretic mobility difference. 3. Catalase migration on starch-gel resembled the human enzyme's electrophoretic mobility for all salmon species and rainbow trout. Catalase activity of the sockeye salmon (2929 +/- 895 mumol min-1 gHb-1) was determined to be lower than human catalase activity. 4. All samples differed from the human enzymes in that they required the presence of a detergent, Triton X-100, for solubilization.

摘要

从几种鲑鱼和虹鳟鱼红细胞中分离出的超氧化物歧化酶显示出单一的活性电泳条带，其向阳极迁移，类似于人类红细胞酶；银大麻哈鱼观察到两条离散条带。2. 30个红大麻哈鱼样本和6个王鲑样本未观察到多态性。只有一个虹鳟鱼样本（12个中的1个）表现出电泳迁移率差异。3. 所有鲑鱼种类和虹鳟鱼的过氧化氢酶在淀粉凝胶上的迁移类似于人类酶的电泳迁移率。红大麻哈鱼的过氧化氢酶活性（2929±895μmol·min⁻¹·gHb⁻¹）被确定低于人类过氧化氢酶活性。4. 所有样本与人类酶的不同之处在于，它们需要去污剂Triton X-100来溶解。