脉冲电场辅助提取九霄塔谷蛋白提取物及其理化性质和生物活性研究。
Pulse electric field assisted process for extraction of Jiuzao glutelin extract and its physicochemical properties and biological activities investigation.
机构信息
Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Key Laboratory of Photochemical Conversion and Optoelectronic Material, Technical Institute of Physics and Chemistry, CAS, Beijing 100190, China.
出版信息
Food Chem. 2022 Jul 30;383:132304. doi: 10.1016/j.foodchem.2022.132304. Epub 2022 Feb 3.
Jiuzao is the residue of baijiu distillation. In this study, pulse electric field (PEF) was used to improve the extraction efficiency of Jiuzao glutelin extract (JGE). The species, physicochemical properties, and biological activities of JGE were investigated to expand its utilization. The results showed that after treatment with PEF under optimal conditions (pulse times, 83 in total; strength, 3.26 kV/cm; Jiuzao/distilled water, 3:20), the JGE content increased by 13.81% compared with ultrasound auxiliary extraction. 59.16% of the JGE was identified to be from sorghum. JGE exhibited desirable foaming, foam stability, water and oil holding capacities, and in vitro antioxidant and angiotensin-converting enzyme inhibitory activities (the IC value was 0.61 mg/mL). In addition, JGE exhibited high cell compatibility at proper concentrations in Caco-2 and CCD 841 CON cells. Overall, PEF is a potential technique to extract high-quality JGE from Jiuzao due to its high yield, efficiency, and maintenance of JGE bioactivities.
酒糟是白酒蒸馏的残渣。本研究采用脉冲电场（PEF）提高酒糟谷朊粉提取（JGE）的提取效率。对 JGE 的种类、理化性质和生物活性进行了研究，以拓展其应用。结果表明，在最优条件（脉冲次数 83 次，强度 3.26 kV/cm，酒糟/蒸馏水 3:20）下用 PEF 处理后，与超声辅助提取相比，JGE 含量增加了 13.81%。59.16%的 JGE 来自高粱。JGE 表现出良好的起泡性、泡沫稳定性、水和油保持能力，以及体外抗氧化和血管紧张素转换酶抑制活性（IC 值为 0.61 mg/mL）。此外，JGE 在 Caco-2 和 CCD 841 CON 细胞中适当浓度下表现出高细胞相容性。总体而言，由于 PEF 具有高得率、高效率和保持 JGE 生物活性的特点，因此是从酒糟中提取高质量 JGE 的一种有潜力的技术。
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