Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng 252059, China; Shandong Liangjian Biotechnology Co., Ltd., Zibo 255000, China.
Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng 252059, China.
Food Chem. 2022 Jul 30;383:132200. doi: 10.1016/j.foodchem.2022.132200. Epub 2022 Jan 22.
Lutein has limited applicability in the food industry because of its poor water solubility and chemical instability. In this study, amorphous and crystalline lutein-loaded microencapsulated powders were prepared via wet media milling and spray drying techniques. The breakage kinetics, surface morphology, physicochemical characteristics, encapsulation efficiency, dissolution behaviour, and storage stability of the two types of microencapsules were determined. Compared with the crystalline formulation, amorphous lutein nanoparticles displayed better breakability (∼478.8 nm within 20 min) in the milling process and faster dissolution rates under both sink and supersaturation conditions (88.0 ± 1.7% and 47.0 ± 3.8%, respectively, within 2 min). Stability testing revealed that the amorphous formulation exhibited slower degradation rates, with decay constants k of 0.03 and 0.07 at 25 and 40 °C, respectively. Our study results suggest that microencapsules with amorphous lutein nanoparticles represent a commercially viable formulation for maintaining chemical stability and improving oral bioavailability.
叶黄素的水溶性差、化学稳定性低，在食品工业中的应用受到限制。本研究采用湿磨和喷雾干燥技术制备了无定形和结晶态叶黄素载微囊粉末。测定了两种微囊的破碎动力学、表面形貌、物理化学特性、包封效率、溶解行为和储存稳定性。与结晶态配方相比，无定形叶黄素纳米颗粒在研磨过程中具有更好的可破碎性（20 min 内达到约 478.8nm），在溶出度实验中，无论是在正常溶解条件还是过饱和条件下，都具有更快的溶解速率（2 min 内分别达到 88.0±1.7%和 47.0±3.8%）。稳定性测试表明，无定形配方的降解速度较慢，在 25 和 40°C 时的衰减常数 k 分别为 0.03 和 0.07。本研究结果表明，含有无定形叶黄素纳米颗粒的微囊是一种有商业应用前景的制剂，可保持化学稳定性，提高口服生物利用度。
