Kuwajima S
Hokkaido Igaku Zasshi. 1986 Jan;61(1):15-23.
Somatic cell hybrids which produced antibodies against measles virus were established by fusing the spleen cells of BALB/c mice immunizing with purified measles virus and the myeloma cell line P3.653 using polyethylene glycol 1,000. Mouse ascites were obtained by inoculating several hybridoma clones, and 2 ascites showed to have high HI titer antibodies. Location of the measles virus antigens on the cells infected with measles virus could be analysed by indirect immunofluorescence technique using these monoclonal antibodies. Reversed passive hemagglutination-inhibition (R-PHI) titers checked by chicken blood cells coated with 2 monoclonal antibodies were parallel with the HI tiers by African green monkey blood cells. These antibodies will be valuable for many virological investigation, for instance, for the detailed antigenic analysis of measles virus and for searching the location of measles virus antigens in the infected cells or tissues.
通过用聚乙二醇1000将用纯化麻疹病毒免疫的BALB/c小鼠的脾细胞与骨髓瘤细胞系P3.653融合，建立了产生抗麻疹病毒抗体的体细胞杂种。通过接种几个杂交瘤克隆获得小鼠腹水，2份腹水显示具有高血凝抑制（HI）效价抗体。使用这些单克隆抗体通过间接免疫荧光技术可以分析麻疹病毒抗原在感染麻疹病毒的细胞上的定位。用2种单克隆抗体包被的鸡血细胞检测的反向被动血凝抑制（R-PHI）效价与用非洲绿猴血细胞检测的HI效价平行。这些抗体对于许多病毒学研究将是有价值的，例如，用于麻疹病毒的详细抗原分析以及用于寻找麻疹病毒抗原在感染细胞或组织中的定位。
