Materials Science and Engineering College, Guilin University of Technology, 541004 Guilin, People's Republic of China.
Dental Clinic and Experimental Center of Medical Sciences, Guilin Medical University, 541002 Guilin, People's Republic of China.
Methods Appl Fluoresc. 2022 Feb 23;10(2). doi: 10.1088/2050-6120/ac5524.
A near-infrared (NIR) light-triggered release method for nitric oxide (NO) was developed utilizing core/shell NaYF: Tm/Yb/Ca@NaGdF: Nd/Yb up-conversion nanoparticles (UCNPs) bearing a mesoporous silica (mSiO) shell loaded with the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). To avoid overheating in biological samples, Ndwas chosen as a sensitizer, Ybions as the bridging sensitizer, and Tmions as UV-emissive activator while co-doping with Cawas done to enhance the luminescence of the activator Tm. NO release from SNAP was triggered by an NIR-UV up-conversion process, initiated by 808 nm light absorbed by the Ndions. NO release was confirmed by the Griess method. Under 808 nm irradiation, the viability of the liver cancer cell line HepG2 significantly decreased with increasing UCNPs@mSiO-SNAP concentration. For a UCNPs@mSiO-SNAP concentration of 200g ml, the cell survival probability was 47%. These results demonstrate that UCNPs@mSiO-SNAP can induce the release of apoptosis-inducing NO by NIR irradiation.
一种近红外（NIR）光触发一氧化氮（NO）释放方法，利用核/壳 NaYF：Tm/Yb/Ca@NaGdF：Nd/Yb 上转换纳米粒子（UCNPs），其表面负载有介孔硅（mSiO）壳，壳中装载有 NO 供体 S-亚硝基-N-乙酰-DL-青霉胺（SNAP）。为避免在生物样本中过热，选择 Nd 作为敏化剂，Yb 离子作为桥接敏化剂，Tm 离子作为紫外发光激活剂，同时共掺杂 Ca 以增强激活剂 Tm 的发光。NO 从 SNAP 的释放是由 808nm 光激发的 NIR-UV 上转换过程触发的，该过程被 Nd 离子吸收。NO 的释放通过格里斯法得到证实。在 808nm 照射下，肝癌细胞系 HepG2 的活力随着 UCNPs@mSiO-SNAP 浓度的增加而显著降低。对于 UCNPs@mSiO-SNAP 浓度为 200μg ml 时，细胞存活率为 47%。这些结果表明，UCNPs@mSiO-SNAP 可以通过近红外照射诱导释放诱导凋亡的 NO。
