Equine Clinic, Internal Medicine, Department of Veterinary Clinical Science (Theuerkauf, Roscher), Institute of Veterinary-Anatomy, -Histology and -Embryology (Obach-Schröck, Staszyk), Clinical Pathophysiology and Veterinary Clinical Pathology, Department of Veterinary Clinical Science (Moritz), Justus-Liebig-University, Giessen, Germany.
J Vet Diagn Invest. 2022 May;34(3):448-457. doi: 10.1177/10406387221077969. Epub 2022 Feb 15.
In humans, activated platelets contribute to sepsis complications and to multiple organ failure. In our prospective analytical study of cases of the equine systemic inflammatory response syndrome (SIRS), we adapted a standard human protocol for the measurement of activated platelets and platelet-leukocyte aggregates (PLAs) in equine platelet-leukocyte-rich plasma (PLRP) by flow cytometry, and we investigated the hypothesis that activated platelets and PLAs are increased in clinical cases of SIRS. We included 17 adult horses and ponies fulfilling at least 2 SIRS criteria, and 10 healthy equids as controls. Activation of platelets was determined by increased expression of CD62P on platelets. Activated platelets and PLAs were measured before and after in vitro activation of platelets with collagen. Median expression of CD62P on platelets was significantly increased after activation in the control group: 1.45% (interquartile range [IQR]: 1.08-1.99%) initially versus 8.78% (IQR: 6.79-14.78%, = 0.002) after activation. The equids with SIRS had significantly more activated platelets and PLAs in native PLRP than controls: CD62P 4.92% (median, IQR: 2.21-12.41%) versus 1.45% in controls (median, IQR: 1.08-1.99%, = 0.0007), and PLAs 4.16% (median, IQR: 2.50-8.58%) versus 2.95% in controls (median, IQR: 1.57-3.22%, = 0.048). To our knowledge, increased platelet activation and PLAs have not been demonstrated previously with flow cytometry in clinical cases of equine SIRS.
在人类中，活化的血小板有助于脓毒症并发症和多器官衰竭。在我们对马全身炎症反应综合征（SIRS）病例的前瞻性分析研究中，我们通过流式细胞术改编了一种用于测量马血小板白细胞丰富血浆（PLRP）中活化血小板和血小板白细胞聚集体（PLA）的标准人类方案，并研究了这样一个假设，即活化血小板和 PLA 在 SIRS 的临床病例中增加。我们纳入了 17 匹符合至少 2 项 SIRS 标准的成年马和小马，以及 10 匹健康的马作为对照。血小板的活化通过血小板上 CD62P 的表达增加来确定。在体外用胶原蛋白激活血小板前后测量活化血小板和 PLA。对照组中血小板 CD62P 的表达在激活后中位数显著增加：初始时为 1.45%（四分位距 [IQR]：1.08-1.99%），激活后为 8.78%（IQR：6.79-14.78%，=0.002）。患有 SIRS 的马与对照组相比，在天然 PLRP 中有更多的活化血小板和 PLA：CD62P 为 4.92%（中位数，IQR：2.21-12.41%），而对照组为 1.45%（中位数，IQR：1.08-1.99%，=0.0007），PLA 为 4.16%（中位数，IQR：2.50-8.58%），而对照组为 2.95%（中位数，IQR：1.57-3.22%，=0.048）。据我们所知，以前没有通过流式细胞术在马 SIRS 的临床病例中证明过血小板活化增加和 PLA。
