Wang Meijia, Tang Kun, Gao Pengfei, Lu Yanjiao, Wang Shanshan, Wu Xiaojie, Zhao Jianping, Xie Jungang
Department of Respiratory and Critical Care Medicine, National Clinical Research Center of Respiratory Disease, Key Laboratory of Pulmonary Diseases of Health Ministry, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Henan University of Science and Technology, Luoyang, China.
J Asthma. 2023 Jan;60(1):203-211. doi: 10.1080/02770903.2022.2040531. Epub 2022 Feb 15.
Club cell 10-kDa protein (CC10) is a documented biomarker for airway obstructive diseases. Primarily produced by nonciliated club cells in the distal airway and in nasal epithelial cells, CC10 suppresses Th2 cell differentiation and Th2 cytokine production. In this study, we aimed to determine whether CC10 can also be used as an alternative biomarker for identifying Type 2 (T2) asthma. 74 patients with asthma, and 24 healthy controls were enrolled in the study. T2-high asthma was defined as elevation in two or more biomarkers, such as sputum eosinophilia ≥ 3%, high blood eosinophils ≥ 300/µL, or high FeNO ≥ 30 ppb. T2-low asthma was defined as no elevation in biomarkers. Enzyme-linked immunosorbent assay (ELISA) was used to assess the CC10 levels in plasma. The plasma CC10 level in patients with T2-high asthma was lower than that of patients with T2-low asthma and healthy controls ( < 0.05). To distinguish between T2-high and T2-low phenotype in patients with asthma, a receiver-operating characteristic (ROC) analysis was performed. It showed a sensitivity of 58.1% and specificity of 78.0% when using 22.74 ng/ml of plasma CC10. Correlation analysis indicated that the plasma CC10 level was inversely correlated with sputum eosinophil, blood eosinophil, and FeNO, and positively correlated with log PD20. However, no correlation with sputum neutrophil percentages, macrophage percentages, IgE, or lung function was found. Plasma CC10 is potentially useful in predicting T2-high and T2-low asthma. Lower plasma CC10 was associated with enhanced airway hyperresponsiveness, and Type 2 inflammation.
克拉拉细胞10 kDa蛋白（CC10）是一种已被证实的气道阻塞性疾病生物标志物。CC10主要由远端气道的无纤毛克拉拉细胞和鼻上皮细胞产生，可抑制Th2细胞分化和Th2细胞因子产生。在本研究中，我们旨在确定CC10是否也可作为识别2型（T2）哮喘的替代生物标志物。74例哮喘患者和24名健康对照者纳入本研究。T2高哮喘定义为两种或更多生物标志物升高，如痰嗜酸性粒细胞≥3%、血嗜酸性粒细胞≥300/µL或呼出一氧化氮（FeNO）≥30 ppb。T2低哮喘定义为生物标志物无升高。采用酶联免疫吸附测定（ELISA）评估血浆中CC10水平。T2高哮喘患者的血浆CC10水平低于T2低哮喘患者和健康对照者（<0.05）。为区分哮喘患者的T2高和T2低表型，进行了受试者工作特征（ROC）分析。当血浆CC10为22.74 ng/ml时，其敏感性为58.1%，特异性为78.0%。相关性分析表明，血浆CC10水平与痰嗜酸性粒细胞、血嗜酸性粒细胞和FeNO呈负相关，与log PD20呈正相关。然而，未发现与痰中性粒细胞百分比、巨噬细胞百分比、免疫球蛋白E（IgE）或肺功能相关。血浆CC10在预测T2高和T2低哮喘方面可能有用。较低的血浆CC10与气道高反应性增强和2型炎症相关。
