Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, India.
Infect Disord Drug Targets. 2022;22(5):e150222201135. doi: 10.2174/1871526522666220215111535.
Superficial Pyogenic Infection (SPI) is the type of a pyogenic infection, which involves the infections of the skin, subcutaneous tissue, and soft tissue. These infections can cause significant morbidity. Antimicrobial Resistance (AMR) has emerged due to the rampant use of broadspectrum agents in superficial pyogenic infections.
The aim of the study was to identify the microbial profile of superficial pyogenic infections and study their antimicrobial resistance.
A cross-sectional observational study was carried out at the Department of Microbiology of a tertiary care hospital in the western region of Rajasthan. Samples included pus, aspirate from the abscess, necrotic tissue, and post-surgical drainage from infected skin at different sites of the patients attending OPD or admitted in IPD and ICU of the hospital. Identification of isolates was carried out by standard bacteriological techniques. The Antibiotic Susceptibility Testing (AST) of bacterial isolates was done by Kirby Bauer disk diffusion method on Mueller Hinton agar (HiMedia, India), and in a few cases, by automated Microscan system as recommended by the Clinical and Laboratory Standards Institute (CLSI), Wayne, USA.
A total of 2283 various specimens were obtained from different areas of healthcare facilities. Pathogenic bacterial isolates were recovered from 303 specimens. Staphylococcus aureus and Escherichia coli were found to be the main offenders. The effective antibiotics for gram-positive isolates were clindamycin, cotrimoxazole, linezolid, tetracycline, and vancomycin, and for gram-negative bacteria, meropenem, imipenem and amikacin were seen to be effective.
This study can help formulate a local antibiotic policy which will restrict the unsupervised antibiotic use and strengthen antibiotic stewardship practices in the hospitals.
浅部化脓性感染（SPI）是一种化脓性感染，涉及皮肤、皮下组织和软组织的感染。这些感染会导致严重的发病率。由于广谱药物在浅部化脓性感染中的广泛应用，出现了抗生素耐药性（AMR）。
本研究旨在确定浅部化脓性感染的微生物谱，并研究其对抗生素的耐药性。
在拉贾斯坦邦西部地区的一家三级保健医院的微生物科进行了一项横断面观察性研究。样本包括来自门诊或住院患者不同部位的脓肿抽吸物、坏死组织和感染皮肤的术后引流物。通过标准细菌学技术进行分离物鉴定。对细菌分离物的抗生素药敏试验（AST）采用 Kirby Bauer 纸片扩散法在 Mueller Hinton 琼脂（印度希麦迪亚公司）上进行，在少数情况下，根据美国临床和实验室标准协会（CLSI）的建议，采用自动 Microscan 系统进行。
从不同的医疗保健设施区域共获得了 2283 份不同的标本。从 303 份标本中回收了病原菌分离物。金黄色葡萄球菌和大肠杆菌被认为是主要的病原体。革兰氏阳性菌分离物的有效抗生素为克林霉素、复方新诺明、利奈唑胺、四环素和万古霉素，革兰氏阴性菌的有效抗生素为美罗培南、亚胺培南和阿米卡星。
本研究可以帮助制定当地的抗生素政策，限制医院中不受监督的抗生素使用，并加强抗生素管理实践。
