Williams D S, Colley N J, Anderson D H, Farber D B, Fisher S K
Invest Ophthalmol Vis Sci. 1986 May;27(5):666-73.
The retina of the lizard, Sceloporus occidentalis, appears to have only cone photoreceptors. Eyecups from this animal were incubated in media containing Earle's Balanced Salts, supplemented with amino acids and vitamins, and gassed with 5% CO2/95% O2. Under these conditions, good morphology, protein synthesis, and normal cyclic AMP and cyclic GMP levels were maintained for 1-2 days. This in vitro preparation is likely to be useful for pharmacological studies of cone photoreceptors.
西部强棱蜥的视网膜似乎仅含有视锥光感受器。将这种动物的眼杯置于含有厄尔平衡盐溶液(添加了氨基酸和维生素)的培养基中,并通入5%二氧化碳/95%氧气进行培养。在这些条件下,良好的形态、蛋白质合成以及正常的环磷酸腺苷(cAMP)和环磷酸鸟苷(cGMP)水平可维持1至2天。这种体外制备方法可能对视锥光感受器的药理学研究有用。