Buttke T M, Pourbohloul S, Miller N W
J Immunol Methods. 1986 May 22;89(2):233-8. doi: 10.1016/0022-1759(86)90363-7.
Methods are described for the efficient induction of primary anti-hapten antibody responses to trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH) in vitro. Mouse leukocytes stimulated in vitro with particulate (bentonite-adsorbed) TNP-KLH demonstrated 10-fold more IgM-secreting, plaque-forming cells (PFC) as measured by a hemolytic plaque assay than did leukocytes stimulated with soluble TNP-KLH. The T dependency of the enhanced antibody response was confirmed using highly enriched B and T lymphocyte populations. These methods should greatly facilitate studies regarding primary in vitro antibody responsiveness to T-dependent antigens.
本文描述了在体外有效诱导针对三硝基苯-钥孔戚血蓝蛋白（TNP-KLH）的原发性抗半抗原抗体反应的方法。通过溶血空斑试验测定，用颗粒状（膨润土吸附的）TNP-KLH体外刺激的小鼠白细胞产生的分泌IgM的空斑形成细胞（PFC）比用可溶性TNP-KLH刺激的白细胞多10倍。使用高度富集的B和T淋巴细胞群体证实了增强的抗体反应对T细胞的依赖性。这些方法应极大地促进关于体外对T细胞依赖性抗原的原发性抗体反应性的研究。
