Yang M C, Miller N W, Clem L W, Buttke T M
Department of Microbiology, University of Mississippi Medical Center, Jackson.
Immunology. 1989 Oct;68(2):181-4.
Albumin-complexed unsaturated fatty acids such as oleic acid (18:1) exerted a dose-dependent inhibitory effect on in vitro primary anti-TNP plaque-forming cell (PFC) responses to trinitrophenyl keyhole limpet haemocyanin (TNP-KLH), but did not affect primary PFC responses to trinitrophenyl lipopolysaccharide (TNP-LPS). The addition of 150 microM 18:1 at the initiation of thymus-dependent (T-D) antibody cultures inhibited the subsequent PFC response by 85%, and removal of the fatty acid after 24 hr did not reverse its inhibitory effect. By contrast, delaying the addition of 18:1 until 3 or 4 days after culture initiation abrogated its inhibitory effects. T-D antibody cultures displayed maximum production of interleukin-2 (IL-2) on the third day after culture initiation and a 24-hr exposure to 18:1 resulted in a dose-dependent inhibition of IL-2 production. Lastly, the addition of exogenous IL-2 reversed the inhibition of PFC responses in cultures transiently exposed to 18:1. These findings suggest that unsaturated fatty acids inhibit in vitro T-D PFC responses by selectively interfering with early stages of the antibody response, particularly those events leading to IL-2 production by T-helper cells.
白蛋白复合的不饱和脂肪酸，如油酸（18:1），对体外针对三硝基苯基钥孔戚血蓝蛋白（TNP-KLH）的初次抗TNP斑块形成细胞（PFC）反应具有剂量依赖性抑制作用，但不影响对三硝基苯基脂多糖（TNP-LPS）的初次PFC反应。在胸腺依赖性（T-D）抗体培养开始时添加150微摩尔的18:1可使随后的PFC反应抑制85%，并且在24小时后去除脂肪酸并不能逆转其抑制作用。相比之下，将18:1的添加延迟到培养开始后3天或4天则可消除其抑制作用。T-D抗体培养在培养开始后第三天显示出白细胞介素-2（IL-2）的最大产量，并且24小时暴露于18:1会导致IL-2产量的剂量依赖性抑制。最后，添加外源性IL-2可逆转短暂暴露于18:1的培养物中PFC反应的抑制。这些发现表明，不饱和脂肪酸通过选择性干扰抗体反应的早期阶段，特别是那些导致辅助性T细胞产生IL-2的事件，来抑制体外T-D PFC反应。
