Keter Mike T, El Halfawy Nancy M, El-Naggar Moustafa Y
Department of Microbiology & Biotechnology, Kenyatta University, 43844-00100, Kenya.
Department of Botany & Microbiology, Faculty of Science, Alexandria University, 21568, Egypt.
Future Microbiol. 2022 Mar;17:325-337. doi: 10.2217/fmb-2021-0053. Epub 2022 Feb 17.
Lactic acid bacteria (LAB) confer beneficial health effects in humans. However, the safety of these bacteria and their potential to spread resistance in the environment must be evaluated. Fifty-three LAB were isolated from different food samples and assessed for the prevalence of virulence determinants and antibiotic resistance profile. Multiple resistance was reported for MIM04, having revealed phenotypic resistance to vancomycin (MIC >128 μg/ml), ampicillin, cefotaxime, oxacillin and gentamicin. Virulence traits (, , and ) were detected using specific primers. CHE32, CHE37 and MLK68 lack virulence genes, possess antimicrobial activity and survive in low pH and bile salt conditions. Isolated LAB revealed probiotic properties.
乳酸菌（LAB）对人类健康有益。然而，必须评估这些细菌的安全性及其在环境中传播耐药性的可能性。从不同食品样本中分离出53株乳酸菌，并评估其毒力决定因素的流行情况和抗生素耐药性谱。据报道，MIM04存在多重耐药性，对万古霉素（MIC>128μg/ml）、氨苄青霉素、头孢噻肟、苯唑西林和庆大霉素表现出表型耐药性。使用特异性引物检测毒力特征（、、和）。CHE32、CHE37和MLK68缺乏毒力基因，具有抗菌活性，能在低pH值和胆盐条件下存活。分离出的乳酸菌显示出益生菌特性。
