Yau J C, Chow H S, Kulkarni S S, Huynh L T, Zander A R
J Clin Lab Immunol. 1986 Jan;19(1):15-8.
This study evaluates the propagation of T lymphocytes in bone marrow cells after in vitro methylprednisolone treatment. Previous studies from this laboratory showed that immunocompetent T lymphocytes could be eliminated from the bone marrow cells by incubation with 15 mg/ml (0.04 M) of methylprednisolone for 1 hr. The effectiveness of the chemoseparation was assessed by E-rosette forming cell assay, mitogen-induced lymphoblastogenic responses, and lymphocyte surface markers. In this study, bone marrow cells treated with different concentrations of methylprednisolone were cultured in the presence of PHA and Interleukin-2. The mitogen-induced lymphoblastogenesis was restored in the 15 mg/ml MP-treated bone marrow cells by the 8th day of culture in the presence of Interleukin-2. The cells in the culture were analyzed by E-rosette forming cell assay. It was shown that the predominant cells in the cultures were E-rosette forming cells. This study demonstrates that immunocompetent T lymphocytes can be regenerated in the presence of mitogen and Interleukin-2 from bone marrow cells treated with 15 mg/ml of methylprednisolone.
本研究评估了体外甲泼尼龙治疗后骨髓细胞中T淋巴细胞的增殖情况。本实验室先前的研究表明，通过与15 mg/ml（0.04 M）甲泼尼龙孵育1小时，可从骨髓细胞中清除免疫活性T淋巴细胞。通过E花环形成细胞试验、丝裂原诱导的淋巴细胞生成反应和淋巴细胞表面标志物评估化学分离的有效性。在本研究中，用不同浓度甲泼尼龙处理的骨髓细胞在PHA和白细胞介素-2存在的情况下进行培养。在白细胞介素-2存在的情况下，培养至第8天时，15 mg/ml甲泼尼龙处理的骨髓细胞中丝裂原诱导的淋巴细胞生成得以恢复。通过E花环形成细胞试验对培养中的细胞进行分析。结果表明，培养中的主要细胞为E花环形成细胞。本研究表明，在有丝裂原和白细胞介素-2存在的情况下，用15 mg/ml甲泼尼龙处理的骨髓细胞可再生免疫活性T淋巴细胞。
