BIRC5 促进喉鳞状细胞癌的进展并预测其预后。
BIRC5 promotes cancer progression and predicts prognosis in laryngeal squamous cell carcinoma.
机构信息
Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountains Areas, School of Life Science of Jiaying University, Meizhou, China.
Henry-Fork School of Food Sciences, Shaoguan University, Shaoguan, China.
出版信息
PeerJ. 2022 Feb 1;10:e12871. doi: 10.7717/peerj.12871. eCollection 2022.
BACKGROUND
Laryngeal squamous cell carcinoma (LSCC) remains one of the most common respiratory tumors worldwide. Baculoviral inhibitor of apoptosis repeat containing 5 ( is a member of the inhibitor-of-apoptosis protein family. BIRC5 plays an important role in various types of cell proliferation, differentiation, migration and invasion. However, the specific role of BIRC5 in LSCC remains unclear.
METHODS
To provide a prognostic biomarker for LSCC, we screened the prognostic genes of LSCC bioinformatics. PPI network and KEGG pathways were used to select hub genes. Clinical prognoses were performed using a Kaplan-Meier plotter and Cox proportional-hazard analysis. BIRC5 expression in LSCC tissues and cell lines were detected by RT-PCR, Western blot and Immunohistochemistry (IHC). Cell proliferation, cell cycle and cell apoptosis were detected with Cell Counting Kit-8 (CCK-8) and Flow Cytometry assay, respectively.
RESULTS
Here, was strongly correlated with higher tumor grade and differentiation. BIRC5 was highly expressed in LSCC tissues when compared with normal tissues and increased expression of BIRC5 was associated with overall survival in LSCC patients. The suppression of BIRC5 induced cell apoptosis and cell cycle arrest, thereby inhibiting the proliferation of LSCC cells. The survival analysis confirmed that higher level of BIRC5 expression predicted poor prognosis of LSCC patients. BIRC5 may act as an oncogene of LSCC development and was suggested as a promising prognostic biomarker for LSCC.
背景
喉鳞状细胞癌（LSCC）仍然是世界上最常见的呼吸道肿瘤之一。杆状病毒凋亡抑制蛋白重复包含 5（BIRC5）是凋亡抑制蛋白家族的一员。BIRC5 在各种类型的细胞增殖、分化、迁移和侵袭中发挥重要作用。然而，BIRC5 在 LSCC 中的具体作用尚不清楚。
方法
为了提供 LSCC 的预后生物标志物，我们对 LSCC 的生物信息学进行了预后基因筛选。使用蛋白质-蛋白质相互作用（PPI）网络和京都基因与基因组百科全书（KEGG）途径选择关键基因。使用 Kaplan-Meier 绘图仪和 Cox 比例风险分析进行临床预后分析。通过 RT-PCR、Western blot 和免疫组织化学（IHC）检测 LSCC 组织和细胞系中的 BIRC5 表达。使用细胞计数试剂盒-8（CCK-8）和流式细胞术分别检测细胞增殖、细胞周期和细胞凋亡。
结果
在这里，与高肿瘤分级和分化相关。与正常组织相比，LSCC 组织中 BIRC5 表达水平较高，BIRC5 表达水平升高与 LSCC 患者的总生存率相关。BIRC5 的抑制诱导细胞凋亡和细胞周期停滞，从而抑制 LSCC 细胞的增殖。生存分析证实，BIRC5 表达水平较高预示着 LSCC 患者预后不良。BIRC5 可能作为 LSCC 发展的癌基因，并被认为是 LSCC 的有前途的预后生物标志物。
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