Pillus L, Solomon F
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2468-72. doi: 10.1073/pnas.83.8.2468.
Most studies of cytoskeletal organelles have concentrated on molecular analyses of abundant and biochemically accessible structures. In many of the classical cases, however, the nature of the system chosen has precluded a concurrent genetic analysis. The mitotic spindle of the yeast Saccharomyces cerevisiae is one example of an organelle that can be studied by both classical and molecular genetics. We show here that this microtubule structure also can be examined biochemically. The spindle can be isolated by selective extractions of yeast cells by using adaptations of methods successfully applied to animal cells. In this way, microtubule-associated proteins of the yeast spindle are identified.
大多数关于细胞骨架细胞器的研究都集中在对丰富且易于进行生化分析的结构进行分子分析。然而，在许多经典案例中，所选系统的性质使得无法同时进行遗传分析。酵母酿酒酵母的有丝分裂纺锤体就是一个可以通过经典遗传学和分子遗传学进行研究的细胞器实例。我们在此表明，这种微管结构也可以进行生化检测。通过采用成功应用于动物细胞的方法对酵母细胞进行选择性提取，可分离出纺锤体。通过这种方式，可鉴定出酵母纺锤体的微管相关蛋白。
