Parrish E P, Garrod D R, Mattey D L, Hand L, Steart P V, Weller R O
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2657-61. doi: 10.1073/pnas.83.8.2657.
Mouse polyclonal antisera were raised to the Mr 130,000 and Mr 115,000 cell surface glycoproteins, desmocollins, of desmosomes from bovine nasal epithelium. Immunoblotting confirmed that the antisera were specific for the desmocollins. An immunofluorescence study showed that the antisera distinguished between the basal and suprabasal layers of bovine and human epidermis. The antibodies reacted with cultured keratinocytes only after calcium-induced stratification. In epidermis, therefore, there appears to be a difference between the desmocollins of basal and suprabasal cells that may be important in relation to epidermal differentiation. Previous work has shown that polyclonal antisera raised in other animals (guinea pigs and rabbits) against desmocollins, as well as against other desmosomal components, react with all desmosome-containing epithelia. In contrast, an immunofluorescence survey of bovine, rat, and human tissues showed that the present mouse antisera stained only suprabasal skin cells and the arachnoid layer of the meninges, demonstrating that these have common determinants that distinguished their desmocollins from those of all other tissues. The antibodies also stained 11 of 12 meningiomas and, therefore, may be useful as a marker not only for the diagnosis of these tumors but also for investigation of their histogenesis.
用来自牛鼻上皮的桥粒中分子量为130,000和115,000的细胞表面糖蛋白桥粒芯蛋白制备了小鼠多克隆抗血清。免疫印迹证实这些抗血清对桥粒芯蛋白具有特异性。免疫荧光研究表明，这些抗血清能够区分牛和人表皮的基底层和基底上层。这些抗体仅在钙诱导分层后才与培养的角质形成细胞发生反应。因此，在表皮中，基底细胞和基底上层细胞的桥粒芯蛋白之间似乎存在差异，这可能与表皮分化有关。先前的研究表明，在其他动物（豚鼠和兔子）中制备的针对桥粒芯蛋白以及其他桥粒成分的多克隆抗血清，可与所有含桥粒的上皮细胞发生反应。相比之下，对牛、大鼠和人类组织的免疫荧光调查显示，目前的小鼠抗血清仅对基底上层皮肤细胞和脑膜的蛛网膜层染色，表明这些组织具有共同的决定簇，使其桥粒芯蛋白与所有其他组织的桥粒芯蛋白不同。这些抗体还对12例脑膜瘤中的11例进行了染色，因此，它们不仅可作为这些肿瘤诊断的标志物，还可用于研究其组织发生。
