[参麦注射液中总人参皂苷的¹H-定量核磁共振分析]

[~1H-qNMR quantification of total ginsenosides in Shenmai Injection].

作者信息

Li Wen-Zhu, Yang Jia-Yu, Zhao Fang, Pan Jian-Yang, Qu Hai-Bin

机构信息

Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China Innovation Center in Zhejiang University, State Key Laboratory of Component-Based Chinese Medicine Hangzhou 310058,China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2022 Feb;47(3):587-592. doi: 10.19540/j.cnki.cjcmm.20211108.302.

DOI:10.19540/j.cnki.cjcmm.20211108.302

PMID:35178940

Abstract

A content determination method based on 1H-qNMR was developed for the determination of total ginsenosides in Shenmai Injection. The parameters were optimized with CD_3OD as the solvent, dimethyl terephthalate as the internal standard, the peak at δ 8.11 as the internal standard peak, and the peaks at δ 1.68 and δ 0.79 as quantitative peaks of total ginsenosides. The developed ~1H-qNMR-based method was validated methodologically. The results showed that the method could achieve accurate measurement of total ginsenosides in Shenmai Injection in the range of 0.167 6-3.091 1 mmol·L(-1). The developed ~1H-qNMR-based method for total ginsenosides is simple in operation, short in analysis time, strong in specificity, independent of accompanying standard curve, and small in sample volume, which can serve as a reliable mean for the quality control of Shenmai Injection. This study is expected to provide new ideas for the development of quantification methods of total ginsenosides.

摘要

建立了一种基于¹H-qNMR的含量测定方法用于测定参麦注射液中总皂苷的含量。以CD₃OD为溶剂、对苯二甲酸二甲酯为内标、δ 8.11处的峰为内标峰、δ 1.68和δ 0.79处的峰为总皂苷的定量峰对参数进行了优化。对所建立的基于¹H-qNMR的方法进行了方法学验证。结果表明该方法能够在0.167 6 - 3.091 1 mmol·L⁻¹范围内准确测定参麦注射液中总皂苷的含量。所建立的基于¹H-qNMR的总皂苷测定方法操作简便、分析时间短、特异性强、无需随行标准曲线且进样量小，可作为参麦注射液质量控制的可靠手段。本研究有望为总皂苷定量方法的发展提供新思路。