Zhang Fang, Lu You-Yuan, Shang Er-Xin, Guo Sheng, Lu Xue-Jun, Ren Gang, Chen Zhan-Ping, Zhao Yu-Ling, Qian Da-Wei, Duan Jin-Ao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization/National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Key Laboratory of Hui Ethnic Medicine Modernization of Ministry of Education, School of Pharmacy, Ningxia Medical University Yinchuan 750021, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jan;47(2):392-402. doi: 10.19540/j.cnki.cjcmm.20211023.102.
Obvious epigenetic differentiation occurred on Lycium barbarum in different cultivation areas in China. To investigate the difference and change rule of DNA methylation level and pattern of L. barbarum from different cultivation areas in China, the present study employed fluorescence-assisted methylation-sensitive amplified polymorphism(MSAP) to analyze the methylation level and polymorphism of 53 genomic DNA samples from Yinchuan Plain in Ningxia, Bayannur city in Inner Mongolia, Jingyuan county and Yumen city in Gansu, Delingha city in Qinghai, and Jinghe county in Xinjiang. The MSAP technical system suitable for the methylation analysis of L. barbarum genomic DNA was established and ten pairs of selective primers were selected. Among amplified 5'-CCGG-3' methylated sites, there were 35.85% full-methylated sites and 39.88% hemi-methylated sites, showing a high degree of epigenetic differentiation. Stoichiometric analysis showed that the ecological environment was the main factor affecting the epigenetic characteristics of L. barbarum, followed by cultivated varieties. Precipitation, air temperature, and soil pH were the main ecological factors affecting DNA methylation in different areas. This study provided a theoretical basis for the analysis of the epigenetic mechanism of L. barbarum to adapt to the diffe-rent ecological environments and research ideas for the introduction, cultivation, and germplasm traceability of L. barbarum.
中国不同栽培地区的枸杞发生了明显的表观遗传分化。为探究中国不同栽培地区枸杞DNA甲基化水平和模式的差异及变化规律，本研究采用荧光辅助甲基化敏感扩增多态性（MSAP）技术，对来自宁夏银川平原、内蒙古巴彦淖尔市、甘肃靖远县和玉门市、青海德令哈市以及新疆精河县的53份基因组DNA样本的甲基化水平和多态性进行分析。建立了适合枸杞基因组DNA甲基化分析的MSAP技术体系，并筛选出10对选择性引物。在扩增出的5'-CCGG-3'甲基化位点中，全甲基化位点占35.85%，半甲基化位点占39.88%，表观遗传分化程度较高。计量分析表明，生态环境是影响枸杞表观遗传特征的主要因素，其次是栽培品种。降水、气温和土壤pH值是影响不同地区DNA甲基化的主要生态因素。本研究为分析枸杞适应不同生态环境的表观遗传机制提供了理论依据，也为枸杞的引种、栽培及种质溯源研究提供了思路。
