利用工程化水纳米结构（EWNS）进行伤口消毒：鲍曼不动杆菌在皮肤上的灭活及生物膜形成抑制的案例研究。
Using engineered water nanostructures (EWNS) for wound disinfection: Case study of Acinetobacter baumannii inactivation on skin and the inhibition of biofilm formation.
作者信息
Vaze Nachiket, Demokritou Philip
机构信息
Center for Nanotechnology and Nanotoxicology, Harvard T.H. Chan School of Public Health, Boston, MA.
Henry Rutgers Chair in Nanoscience and Environmental Bioengineering at the Rutgers School of Public Health and Environmental and Occupational Health Sciences Institute, Piscataway, NJ.
出版信息
Nanomedicine. 2022 Jun;42:102537. doi: 10.1016/j.nano.2022.102537. Epub 2022 Feb 16.
Engineered water nanostructures (EWNS) were utilized to deliver a cocktail of nature derived antimicrobials, to assess their efficacy as a solution to the problem of wound infections. The wound related microorganism Acinetobacter baumannii was inoculated on stainless steel and porcine skin and treated with EWNS. EWNS were able to reduce A. baumannii on stainless steel by 4.79 logs in 15 min, and 2 logs in 30 min on porcine skin. The EWNS were able to reduce the strength of A. baumannii biofilm on stainless steel by 87.31% as measured with the XTT assay (P < .001) and 86.27% in cellular counts (P < .001), after two EWNS interventions of 30 min each. Total antimicrobial dose delivered to the surface was 1.42 ng. SEM of biofilms after EWNS treatment showed reduced biomass. These results indicate that the EWNS technology has potential for application in field of wound disinfection and healing.
工程化水纳米结构（EWNS）被用于递送一组天然来源的抗菌剂，以评估其作为解决伤口感染问题的一种解决方案的功效。将与伤口相关的微生物鲍曼不动杆菌接种在不锈钢和猪皮上，并用EWNS进行处理。EWNS能够在15分钟内使不锈钢上的鲍曼不动杆菌减少4.79个对数级，在猪皮上30分钟内减少2个对数级。通过XTT测定法测量，经过两次每次30分钟的EWNS干预后，EWNS能够使不锈钢上鲍曼不动杆菌生物膜的强度降低87.31%（P <.001），细胞计数降低86.27%（P <.001）。递送至表面的总抗菌剂量为1.42纳克。EWNS处理后生物膜的扫描电子显微镜显示生物量减少。这些结果表明，EWNS技术在伤口消毒和愈合领域具有应用潜力。
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