Gu Zefeng, Yang Jingmiao, Yang Min, Jiao Yu
Fishery College, Guangdong Ocean University, Zhanjiang, 524088, China.
Fishery College, Guangdong Ocean University, Zhanjiang, 524088, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China; Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Zhanjiang, 524088, China.
Fish Shellfish Immunol. 2022 Apr;123:10-19. doi: 10.1016/j.fsi.2022.02.030. Epub 2022 Feb 16.
Pre-grafting condition is an important method to promote recovery from transplant surgery during pearl production. In the present study, we constructed two DNA methylomes from pearl oysters with and without conditioning to investigate the molecular mechanism of the pearl oyster Pinctada fucata martensii underlying the pre-grafting condition. A total of 4,594,997 and 4,930,813 methyl CG in the control (Con) and pre-grafting group (PT) were detected, resulting in the whole genome methylation profile and methylation pattern in P. f. martensii. Results reveal that the promoter, especially the CpG island-rich region, was more infrequently methylated than the gene function elements in P. f. martensii. A total of 51,957 differently methylated regions (DMRs) between Con and PT were obtained, including 3789 DMR in the promoter and 16,021 in the gene body. Based on gene ontology and pathway enrichment analyses, these DMRs were mainly related to "cellular process", "metabolic process", "Epstein-Barr virus infection", and "Fanconi anemia pathway". The methylation site in the promoter region may be associated with the promoter activity and transcription factor binding. These results help our understanding of the mechanism of pre-grafting condition, thereby providing key information in guiding to improve the conditioning methods for enhanced pearl oyster survival rate after transplantation.
植核前处理是珍珠生产过程中促进移植手术恢复的重要方法。在本研究中，我们构建了经过和未经过预处理的合浦珠母贝的两个DNA甲基化组，以研究合浦珠母贝在植核前处理背后的分子机制。在对照组（Con）和植核前处理组（PT）中分别检测到4,594,997个和4,930,813个甲基化的CG，从而得到了合浦珠母贝的全基因组甲基化图谱和甲基化模式。结果显示，在合浦珠母贝中，启动子，尤其是富含CpG岛的区域，甲基化频率低于基因功能元件。在Con和PT之间共获得了51,957个差异甲基化区域（DMR），其中包括3789个启动子区域的DMR和16,021个基因体区域的DMR。基于基因本体论和通路富集分析，这些DMR主要与“细胞过程”、“代谢过程”、“爱泼斯坦-巴尔病毒感染”和“范可尼贫血通路”相关。启动子区域的甲基化位点可能与启动子活性和转录因子结合有关。这些结果有助于我们理解植核前处理的机制，从而为指导改进预处理方法以提高移植后合浦珠母贝的存活率提供关键信息。
