Talpo Tassiana Cristina, Motta Bruno Pereira, Oliveira Juliana Oriel de, Figueiredo Ingrid Delbone, Pinheiro Camila Graça, Dos Santos Carlos Henrique Corrêa, Carvalho Mário Geraldo de, Brunetti Iguatemy Lourenço, Baviera Amanda Martins
São Paulo State University (Unesp), School of Pharmaceutical Sciences, Department of Clinical Analysis, Araraquara, São Paulo, Brazil.
Federal Rural University of Rio de Janeiro (UFRRJ), Institute of Exact Sciences, Department of Chemistry, Seropédica, Rio de Janeiro, Brazil.
Obes Res Clin Pract. 2022 Mar-Apr;16(2):130-137. doi: 10.1016/j.orcp.2022.02.004. Epub 2022 Feb 17.
Obesity is accompanied by insulin resistance and glucose intolerance, which favor the onset of complications related to oxidative stress. The aim of this study was to investigate the effects and underlying mechanisms of hydroethanolic extract from Siolmatra brasiliensis stems on insulin resistance, glucose intolerance, advanced glycation end product (AGE) formation, and oxidative stress in mice with induced obesity.
C57BL-6 J mice were fed a high-fat diet for 14 weeks and treated with 125 or 250 mg/kg S. brasiliensis extract during the last 7 weeks. The study assessed glucose tolerance and insulin sensitivity, lipid profile, plasma levels of thiobarbituric acid reactive substances (TBARS, biomarkers of oxidative damage), fluorescent AGEs (biomarkers of advanced glycation), and paraoxonase 1 (PON1) activity (antioxidant enzyme). The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the liver and kidneys were also investigated.
Siolmatra brasiliensis extract had antiobesogenic effects; improved insulin sensitivity and glucose tolerance; decreased the total plasma cholesterol levels; decreased the levels of glycoxidative stress biomarkers, including AGEs (plasma, liver, kidneys) and TBARS (liver, kidneys); and also improved endogenous antioxidant defenses by increasing the activities of PON1 (plasma), SOD (kidneys), CAT (liver, kidneys), and GSH-Px (kidneys).
This study expands on our knowledge about the pharmacological properties of S. brasiliensis and substantiates the potential of this plant species to be used as a complementary therapeutic agent to alleviate the metabolic dysfunctions resulting from dyslipidemia and glycoxidative stress.
肥胖伴随着胰岛素抵抗和葡萄糖不耐受，这有利于与氧化应激相关并发症的发生。本研究的目的是探讨巴西西奥拉特拉茎部水乙醇提取物对诱导肥胖小鼠的胰岛素抵抗、葡萄糖不耐受、晚期糖基化终产物（AGE）形成和氧化应激的影响及潜在机制。
C57BL-6 J小鼠喂食高脂饮食14周，并在最后7周用125或250 mg/kg巴西西奥拉特拉提取物进行处理。该研究评估了葡萄糖耐量和胰岛素敏感性、血脂谱、硫代巴比妥酸反应性物质（TBARS，氧化损伤生物标志物）的血浆水平、荧光AGEs（晚期糖基化生物标志物）和对氧磷酶1（PON1）活性（抗氧化酶）。还研究了肝脏和肾脏中抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。
巴西西奥拉特拉提取物具有抗肥胖作用；改善了胰岛素敏感性和葡萄糖耐量；降低了总血浆胆固醇水平；降低了糖氧化应激生物标志物的水平，包括AGEs（血浆、肝脏、肾脏）和TBARS（肝脏、肾脏）；还通过增加PON1（血浆）、SOD（肾脏）、CAT（肝脏、肾脏）和GSH-Px（肾脏）的活性改善了内源性抗氧化防御。
本研究扩展了我们对巴西西奥拉特拉药理特性的认识，并证实了该植物物种作为辅助治疗剂缓解血脂异常和糖氧化应激导致的代谢功能障碍的潜力。
