Centre for Neuroscience Research (NeuRon), Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia.
School of Medicine, International Medical University, Kuala Lumpur, Malaysia.
Curr Eye Res. 2022 Jun;47(6):866-873. doi: 10.1080/02713683.2022.2033270. Epub 2022 Mar 22.
Retinal and optic nerve damage in glaucoma involves excitotoxicity via N-methyl-D-aspartate (NMDA) receptors. Since, -resveratrol (TR) is known to provide neuroprotection, we investigated its protective effects against NMDA-induced retinal and optic nerve injury.
Sprague Dawley rats were divided into four groups which received vehicle (PBS), NMDA, and TR 0.4 or TR 4 nmol 24 h prior to NMDA, unilaterally and intravitreally. Seven days post-injection, rats were euthanized; eyeballs were enucleated and subjected to hematoxylin and eosin and terminal transferase dUTP nick end labeling staining while optic nerves were isolated for toluidine blue staining.
Retinal morphometry showed that ganglion cell layer (GCL) layer thickness within inner retina (IR), retinal cell count (RCC) per 100-µm length of GCL, RCC per 100-µm area of GCL, and RCC per 100 µm of IR were significantly higher in both TR-treated groups compared to the NMDA group. No differences were observed between the two dose groups. Optic nerve morphology was in accordance with the retinal morphology whereby TR-treated groups showed significantly lesser degenerative changes compared to NMDA-treated group.
TR protects against NMDA-induced changes in retinal and optic nerve morphology by preventing retinal cell apoptosis.
青光眼导致的视网膜和视神经损伤涉及 N-甲基-D-天冬氨酸（NMDA）受体的兴奋性毒性。由于白藜芦醇（TR）已知具有神经保护作用，我们研究了其对 NMDA 诱导的视网膜和视神经损伤的保护作用。
将 Sprague Dawley 大鼠分为四组，分别在 NMDA 前 24 小时经玻璃体单侧给予载体（PBS）、NMDA 和 TR 0.4 或 4 nmol。注射后 7 天处死大鼠；眼球被取出并进行苏木精和伊红以及末端转移酶 dUTP 缺口末端标记染色，同时视神经被分离用于甲苯胺蓝染色。
视网膜形态计量学显示，内视网膜（IR）中的神经节细胞层（GCL）厚度、GCL 每 100-µm 长度的视网膜细胞计数（RCC）、GCL 每 100-µm 面积的 RCC 和每 100 µm 的 IR 中的 RCC 在两种 TR 治疗组中均明显高于 NMDA 组。两个剂量组之间没有差异。视神经形态与视网膜形态一致，TR 治疗组显示出与 NMDA 治疗组相比明显较少的退行性变化。
TR 通过防止视网膜细胞凋亡来对抗 NMDA 诱导的视网膜和视神经形态变化。
