Sharp P J, Sterling R J, Milton R C, Millar R P
Br Poult Sci. 1986 Mar;27(1):129-35. doi: 10.1080/00071668608416864.
The ability of synthetic vertebrate luteinising hormone releasing hormones (LHRHs) and their long-acting analogues to maintain elevated plasma luteinising hormone (LH) concentrations and to stimulate ovarian growth was investigated in incubating bantam hens. Chicken LHRH-II (pGlu1-His2-Trp3-Ser4-His5-Gly6-Trp7-Tyr8-Pro9-G ly10-NH2) was more effective than chicken LHRH-I (pGlu1-His2-Trp3-Ser4-Tyr5-Gly6-Leu7-Gln8-Pro9-Gly10-N H2) or porcine LHRH (pGlu1-His2-Trp3-Ser4-Tyr5-Gly6-Leu7-Arg8-Pro9-Gly10-N H2) in stimulating the release of LH. Long-acting analogues of chicken LHRHs (chLHRHs) were created by substituting D-amino acids in position 6. An intravenous injection (10 micrograms/bird) of D-Arg6-chLHRH-II or of a long-acting mammalian analogue of LHRH (buserelin) resulted in a sustained release of LH for up to 8 h. Less sustained releases of LH were observed after the same doses of D-Ala6-chLHRH-I or of D-Trp6-chLHRH-I. Repeated subcutaneous injections of D-Arg6-chLHRH-II or buserelin at 7 to 9 h intervals for 9 d resulted in loss of pituitary gland responsiveness to these analogues. For this reason, the treatment failed to maintain elevated plasma LH concentrations and did not stimulate the growth of the ovary or oviduct.
在孵化中的矮脚鸡身上研究了合成脊椎动物促黄体生成激素释放激素（LHRHs）及其长效类似物维持血浆促黄体生成激素（LH）浓度升高和刺激卵巢生长的能力。鸡促黄体生成激素释放激素-II（pGlu1-His2-Trp3-Ser4-His5-Gly6-Trp7-Tyr8-Pro9-Gly10-NH2）在刺激LH释放方面比鸡促黄体生成激素释放激素-I（pGlu1-His2-Trp3-Ser4-Tyr5-Gly6-Leu7-Gln8-Pro9-Gly10-NH2）或猪促黄体生成激素释放激素（pGlu1-His2-Trp3-Ser4-Tyr5-Gly6-Leu7-Arg8-Pro9-Gly10-NH2）更有效。通过在第6位取代D-氨基酸来制备鸡促黄体生成激素释放激素（chLHRHs）的长效类似物。静脉注射（10微克/只）D-Arg6-chLHRH-II或长效促黄体生成激素释放激素的哺乳动物类似物（布舍瑞林）导致LH持续释放长达8小时。相同剂量的D-Ala6-chLHRH-I或D-Trp6-chLHRH-I注射后观察到LH的持续释放较少。以7至9小时间隔重复皮下注射D-Arg6-chLHRH-II或布舍瑞林9天导致垂体对这些类似物的反应性丧失。因此，该治疗未能维持血浆LH浓度升高，也未刺激卵巢或输卵管生长。
