Potu Hemanth, F A Lali, K Anilkumar, M T Dipu, T V Aravindakshan
Department of Animal Genetics and Breeding, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, India.
Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, Kerala Veterinary and Animal Sciences University, Thrissur, India.
Anim Biotechnol. 2023 Nov;34(4):1544-1552. doi: 10.1080/10495398.2022.2039169. Epub 2022 Feb 21.
gene is located in the neighborhood of a quantitative trait loci for milk production in bovine autosome 23. We verified the genetic variability of exon-3 in and its association with milk production traits in Holstein Friesian crossbreds of Kerala. Genomic DNA was isolated and 94bp fragment enclosing exon-3 was amplified by primers designed using PRIMER 3 based on reference sequence (GenBank NC_037350). Pooled amplicons were sequenced by Sanger's method and a novel single nucleotide polymorphism due to a transversion of guanine to adenine at position 21 of amplicon (G21A) leading to amino acid change arginine to glutamine was detected. The study population was genotyped by high-resolution melt curve analysis and revealed two genotypes with frequencies GG/0.84 and GA/0.14. The allele G was found to be the major one (G/0.93 and A/0.07). Moreover, association analysis of G21A with milk production traits was done using the General linear model-Analysis of Variance considering herd, season, and parity as non-genetic factors and milk production trait as a dependent variable. In analysis, animals with GA genotype were found to be having significantly higher ( ≤ 0.01) 305 day milk (GG:2720.74 ± 122.92 kg; GA:3250.20 ± 183.24 kg), fat (GG:106.55 ± 4.32 kg; GA:126.30 ± 13.35 kg), and SNF yield (GG: 211.52 ± 9.20 kg; GA: 246.90 ± 13.70 kg). However, GG (7.80 ± 0.04) genotype has significantly higher ( ≤ 0.05) SNF percent than GA (7.65 ± 0.07). Butyrophilin gene polymorphism G21A can be suggested as a molecular marker for future breeding programmes of cattle.
基因位于牛常染色体23上一个与产奶量相关的数量性状基因座附近。我们验证了喀拉拉邦荷斯坦弗里生杂交牛中外显子3的遗传变异性及其与产奶性状的关联。提取基因组DNA，基于参考序列（GenBank NC_037350）使用PRIMER 3设计引物，扩增出包含外显子3的94bp片段。混合扩增产物采用桑格法测序，检测到一个新的单核苷酸多态性，扩增产物第21位鸟嘌呤颠换为腺嘌呤（G21A），导致氨基酸由精氨酸变为谷氨酰胺。通过高分辨率熔解曲线分析对研究群体进行基因分型，发现两种基因型，频率分别为GG/0.84和GA/0.14。发现等位基因G为主要等位基因（G/0.93和A/0.07）。此外，使用一般线性模型-方差分析，将牛群、季节和胎次作为非遗传因素，产奶性状作为因变量，对G21A与产奶性状进行关联分析。分析发现，GA基因型的动物305天产奶量（GG：2720.74±122.92千克；GA：3250.20±183.24千克）、脂肪量（GG：106.55±4.32千克；GA：126.30±13.35千克）和非脂固形物产量（GG：211.52±9.20千克；GA：246.90±13.70千克）显著更高（≤0.01）。然而，GG（7.80±0.04）基因型的非脂固形物百分比显著高于GA（7.65±0.07）（≤0.05）。嗜乳脂蛋白基因多态性G—21A可作为未来牛育种计划的分子标记。
