Leite Ferreira Laís, Pereira Generoso Laura, Medeiros Ana Carolina, de Medeiros Priscila, Leonardo de Freitas Renato, Lourenço da Silva Marcelo, Resende Torres da Silva Josie
Motricity Sciences Institute, UNIFAL-MG, Alfenas, MG, Brazil; Postgraduate Program in Biosciences Applied to Health (PPGB), UNIFAL, Alfenas, MG, Brazil.
Motricity Sciences Institute, UNIFAL-MG, Alfenas, MG, Brazil; Graduation in Physiotherapy at the Motricity Sciences Institute, UNIFAL, Alfenas, MG, Brazil.
Behav Brain Res. 2022 Apr 29;424:113803. doi: 10.1016/j.bbr.2022.113803. Epub 2022 Feb 18.
Morphological reorganization in the neural networks of the medial prefrontal cortex (mPFC) may be involved in the development of chronic neuropathic pain (NP).
We investigated whether inactivation and neurostimulation of the infralimbic division (IFL) of the mPFC alter electroacupuncture-induced analgesia (EIA) at 2 Hz and 2/100 Hz in animals with chronic NP.
Wistar rats were submitted to chronic constrictor injury of the ischiadicus nerve (CCI). Von Frey and acetone tests were performed to evaluate mechanical or cold allodynia. Animals were submitted to electroacupuncture (EA) at 2 Hz and 2/100 Hz for 20 min. After EA, the IFL cortex synaptic contacts were inactivated by cobalt chloride (200 nL of 1.0 mM CoCl). Neurostimulation of the IFL cortex was also performed at 20 µA for 15 s, after EA, using a deep brain stimulation device.
EA at 2 Hz and 2/100 Hz attenuated mechanical or cold allodynia in CCI rats. Microinjection of CoCl into the IFL division of the mPFC blocked the EA effect. EA at 2 Hz and 2/100 Hz, in association with neurostimulation of the IFL cortex, attenuated mechanical and thermal allodynia.
EA induces antinociception in CCI rats. The analgesia was potentiated in association with neurostimulation in the IFL division of the mPFC.
内侧前额叶皮质（mPFC）神经网络的形态重组可能参与慢性神经性疼痛（NP）的发展。
我们研究了mPFC腹内侧区（IFL）的失活和神经刺激是否会改变慢性NP动物在2Hz和2/100Hz频率下的电针诱导镇痛（EIA）。
将Wistar大鼠进行坐骨神经慢性缩窄损伤（CCI）。采用von Frey和丙酮试验评估机械性或冷觉异常性疼痛。动物接受2Hz和2/100Hz频率的电针治疗20分钟。电针治疗后，用氯化钴（200nL 1.0mM CoCl）使IFL皮质突触联系失活。电针治疗后，还使用深部脑刺激装置以20µA的电流对IFL皮质进行15秒的神经刺激。
2Hz和2/100Hz频率的电针减轻了CCI大鼠的机械性或冷觉异常性疼痛。向mPFC的IFL区微量注射CoCl可阻断电针的效果。2Hz和2/100Hz频率的电针联合IFL皮质的神经刺激减轻了机械性和热觉异常性疼痛。
电针可诱导CCI大鼠产生抗伤害感受。mPFC的IFL区神经刺激可增强这种镇痛作用。
