Adam W R, Culvenor A J, Hall J, Jarrott B, Wellard R M
Clin Exp Pharmacol Physiol. 1986 Jan;13(1):47-53. doi: 10.1111/j.1440-1681.1986.tb00314.x.
Utilizing a mono-specific antiserum produced in rabbits to hog kidney aromatic L-amino acid decarboxylase (AADC), the enzyme was localized in rat kidney by immunoperoxidase staining. AADC was located predominantly in the proximal convoluted tubules; there was also weak staining in the distal convoluted tubules and collecting ducts. An increase in dietary potassium or sodium intake produced no change in density or distribution of AADC staining in kidney. An assay of AADC enzyme activity showed no difference in cortex or medulla with chronic potassium loading. A change in distribution or activity of renal AADC does not explain the postulated dopaminergic modulation of renal function that occurs with potassium or sodium loading.
利用在兔体内产生的针对猪肾芳香族L-氨基酸脱羧酶（AADC）的单特异性抗血清，通过免疫过氧化物酶染色将该酶定位在大鼠肾脏中。AADC主要位于近端曲管；在远端曲管和集合管中也有弱阳性染色。饮食中钾或钠摄入量的增加并未使肾脏中AADC染色的密度或分布发生变化。AADC酶活性测定显示，慢性钾负荷情况下，皮质或髓质无差异。肾脏AADC分布或活性的变化并不能解释钾或钠负荷时推测的肾脏功能多巴胺能调节。
