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长链非编码 RNA FGD5-AS1 通过影响 miR-296-5p/STAT3 轴调控激素诱导性股骨头坏死骨髓间充质干细胞增殖和凋亡。

lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head.

机构信息

Laboratory, Shandong First Medical University, Shandong Academy of Medical Sciences, Tai'an 271016, Shandong Province, China.

Department of Orthopedics, The Second Affiliated Hospital of Shandong First Medical University, Tai'an 271000, Shandong Province, China.

出版信息

J Healthc Eng. 2022 Feb 12;2022:9364467. doi: 10.1155/2022/9364467. eCollection 2022.

DOI:10.1155/2022/9364467
PMID:35190765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8858055/
Abstract

BACKGROUND

Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH.

METHODS

The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay.

RESULTS

Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs.

CONCLUSION

lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.

摘要

背景

股骨头坏死（ONFH）是一种常见的髋关节疾病，危害较大，严重影响患者的生活。本研究旨在阐明 lncRNA FGD5-AS1 在 ONFH 中的调控机制。

方法

采用 RT-qPCR 和 Western blot 检测蛋白和 mRNA 的表达。通过双荧光素酶报告实验、CCK-8 检测和流式细胞术检测 lncRNA FGD5-AS1 的调控机制。

结果

地塞米松（Dex）可呈剂量依赖性抑制 hBMSCs 的增殖和分化，并诱导其凋亡。lncRNA FGD5-AS1 的过表达可促进 Dex 处理的 hBMSCs 的增殖并抑制其凋亡。此外，lncRNA FGD5-AS1 可作为 miR-296-5p 的海绵。此外，miR-296-5p 可直接靶向 STAT3。更重要的是，miR-296-5p 和 STAT3 可影响 lncRNA FGD5-AS1 在 Dex 处理的 hBMSCs 中的功能。

结论

lncRNA FGD5-AS1 通过作为 miR-296-5p 的海绵和上调 STAT3 促进激素诱导的 ONFH 中的细胞增殖并抑制凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/2165d2ad9095/JHE2022-9364467.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/a94261967774/JHE2022-9364467.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/446d98124186/JHE2022-9364467.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/134705a74811/JHE2022-9364467.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/5993a274737b/JHE2022-9364467.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/2165d2ad9095/JHE2022-9364467.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/a94261967774/JHE2022-9364467.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/446d98124186/JHE2022-9364467.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/134705a74811/JHE2022-9364467.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/5993a274737b/JHE2022-9364467.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ab4/8858055/2165d2ad9095/JHE2022-9364467.005.jpg

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