基于硫化银量子点和硅纳米粒子的双发射比率型纳米探针测定氰化物。

Determination of cyanide based on a dual-emission ratiometric nanoprobe using silver sulfide quantum dots and silicon nanoparticles.

机构信息

Research Laboratory of Nanoporous Materials, Faculty of Chemistry, Iran University of Science and Technology, Narmak, 16846-13114, Tehran, Iran.

出版信息

Mikrochim Acta. 2022 Feb 22;189(3):115. doi: 10.1007/s00604-022-05209-5.

DOI:10.1007/s00604-022-05209-5

PMID:35192072

Abstract

A novel ratiometric fluorescent nanoprobe was designed for the sensitive determination of cyanide anion (CN) by the electrostatic attraction between positively charged silicon nanoparticles (Si NPs) and negatively charged silver sulfide quantum dots (AgS QDs). The nanoprobe exhibited two well-resolved emission peaks at 446 nm and 540 nm under a single excitation wavelength (360 nm). In the presence of CN, the fluorescence of AgS QDs at 540 nm was remarkably quenched, while the fluorescence of the Si NPs at 446 nm remained constant, establishing the desired conditions for ratiometric fluorescence detection. Under optimal conditions, the ratiometric fluorescence assay showed good linearity (R = 0.9921) within the range 0.05-15 μM, and the limit of detection was calculated to be 56 nM (at an S/N ratio of 3). The proposed AgS QD/Si NP nanoprobe has been successfully used to determine CN in water and sprouting potato samples with satisfactory recoveries in the range 97-110.5%.

摘要

一种新型的比率荧光纳米探针被设计用于通过带正电荷的硅纳米颗粒（Si NPs）和带负电荷的硫化银量子点（AgS QDs）之间的静电吸引来灵敏地测定氰根阴离子（CN）。该纳米探针在单一激发波长（360nm）下显示出两个分辨率良好的发射峰，分别位于 446nm 和 540nm。在存在 CN 的情况下，AgS QDs 在 540nm 处的荧光显著猝灭，而 Si NPs 在 446nm 处的荧光保持不变，从而建立了比率荧光检测所需的条件。在最佳条件下，比率荧光分析在 0.05-15μM 范围内表现出良好的线性（R=0.9921），检测限计算为 56nM（信噪比为 3）。所提出的 AgS QD/Si NP 纳米探针已成功用于测定水样和发芽马铃薯样品中的 CN，回收率在 97-110.5%范围内令人满意。