Camel Research Center, King Faisal University, Al-Hasa, Saudi Arabia.
Veterinary Teaching Hospital, King Faisal University Al-Hasa, Saudi Arabia.
J Infect Dev Ctries. 2022 Jan 31;16(1):134-146. doi: 10.3855/jidc.15023.
Members of the Corynebacterium cystitidis species are usually isolated from kidney and urine of cow having pyelonephritis. Nevertheless, we have isolated Corynebacterium cystitidis for the first time from uterus of camels, extending the type of mammalian host for this species. Furthermore, it remains unknown whether there are significant genetic variations between strains isolated from different host species and anatomic sites. In this perspective, we investigated the genomic diversity of Corynebacterium cystitidis species, whose pan genome remain unexplored to date.
Thus, we sequenced and compared the genomes of five Corynebacterium cystitidis of camel origin and a public genome of cow associated Corynebacterium cystitidis.
Results revealed open pan genome of 4,038 gene clusters and horizontal gene transfer played a role in the extensive genetic diversity. Further, we found an obvious distinction between cow and camel associated C. cystitidis via phylogenomic analysis and by average nucleotide identity value of 95% between the two distant lineages and > 99% within camel associated C. cystitidis strains. Moreover, our data supports the hypothesis that the gene repertoire of cow associated Corynebacterium cystitidis developed so as to become more adaptable to the urine milieu. These genetic potentials are specifically evident for genes required for benzoate breakdown, iron transport, citrate and alanine utilization.
Our findings confirm the differentiation of strains into camel lineage and cow lineage. These different niches, comprising the uterus of camel and urinary tract of cow probably played a role in shaping the gene repertoire of strains.
棒状杆菌属膀胱炎物种的成员通常从患有肾盂肾炎的牛的肾脏和尿液中分离出来。然而，我们首次从骆驼的子宫中分离出膀胱炎棒状杆菌，扩大了该物种的哺乳动物宿主类型。此外，目前尚不清楚是否存在不同宿主物种和解剖部位分离的菌株之间存在显着的遗传变异。从这个角度来看，我们研究了膀胱炎棒状杆菌物种的基因组多样性，迄今为止，其泛基因组仍未得到探索。
因此，我们对 5 株骆驼来源的膀胱炎棒状杆菌和 1 株公开的牛相关膀胱炎棒状杆菌的基因组进行了测序和比较。
结果显示，开放的泛基因组包含 4038 个基因簇，水平基因转移在广泛的遗传多样性中发挥了作用。此外，通过系统发育基因组分析和两种不同谱系之间的平均核苷酸同一性值为 95%，以及骆驼相关膀胱炎棒状杆菌菌株内的>99%，我们发现牛和骆驼相关 C. cystitidis 之间存在明显区别。此外，我们的数据支持了以下假设，即牛相关膀胱炎棒状杆菌的基因库的发展是为了使其更适应尿液环境。这些遗传潜力在用于苯甲酸分解、铁运输、柠檬酸和丙氨酸利用的基因中尤为明显。
我们的研究结果证实了菌株分为骆驼谱系和牛谱系的分化。这些不同的生态位，包括骆驼的子宫和牛的泌尿道，可能在塑造菌株的基因库方面发挥了作用。
