Kaiho M, Anzai M, Mukaida M, Ishiyama I
Histochemistry. 1986;84(2):151-3. doi: 10.1007/BF00499826.
The myoglobin content of chicken myocardial cells was studied using indirect-immunoperoxidase histochemistry. While ordinary myocardial cells exhibited a homogeneous reaction pattern, the reactions for ventricular Purkinje fibers were remarkably heterogeneous. On the basis of the degree of staining, three types of cells, i.e., dark, intermediate, and clear, were distinguishable. In addition, the cytological heterogeneity of Purkinje cells was confirmed using conventional and immunological electron microscopy. The dark cells contained more myofibrils, mitochondria, and other organelles (e.g., ribosomes) than the clear cells.
采用间接免疫过氧化物酶组织化学法研究了鸡心肌细胞的肌红蛋白含量。普通心肌细胞呈现均匀的反应模式，而心室浦肯野纤维的反应则明显不均一。根据染色程度，可区分出三种类型的细胞，即深色细胞、中间色细胞和浅色细胞。此外，通过传统电子显微镜和免疫电子显微镜证实了浦肯野细胞的细胞学异质性。深色细胞比浅色细胞含有更多的肌原纤维、线粒体和其他细胞器（如核糖体）。
