van de Klundert J A, van Gestel M H, van Doorn E, Mouton R P
J Antimicrob Chemother. 1986 Apr;17(4):471-9. doi: 10.1093/jac/17.4.471.
The incorporation of beta-lactamases from crude, cell-free lysates incorporated into agar plates used for antibiotic sensitivity testing with sensitivity discs results in a characteristic decrease in the inhibition zones. The degree of zone reduction for a particular antibiotic depends on the substrate specificity of the enzyme involved and the concentration of the enzyme in the agar. By using this technique a distinction can be made between cephalosporinases, penicillinases and broad-spectrum enzymes. In a number of cases, the identification of the enzyme subclass is possible, provided that the relevant penicillin and cephalosporin discs are included in the test. For the elucidation of substrate profiles for routine and epidemiological purposes this method can replace other, more elaborate techniques, such as the iodometric, spectrophotometric and acidimetric methods. An analysis by this method is presented for 126, unrelated, consecutive clinical isolates.
将来自粗制无细胞裂解物的β-内酰胺酶掺入用于抗生素敏感性测试的琼脂平板中，使用敏感性纸片会导致抑菌圈出现特征性减小。特定抗生素的抑菌圈减小程度取决于所涉及酶的底物特异性以及琼脂中酶的浓度。通过使用该技术，可以区分头孢菌素酶、青霉素酶和广谱酶。在许多情况下，只要测试中包含相关的青霉素和头孢菌素纸片，就有可能鉴定酶的亚类。为了阐明常规和流行病学目的的底物谱，该方法可以替代其他更复杂的技术，如碘量法、分光光度法和酸碱滴定法。本文介绍了对126株不相关的连续临床分离株采用该方法进行的分析。
