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使用单克隆抗体T101试剂盒进行T细胞检测。

T-cell detection with monoclonal antibody T101 kits.

作者信息

Pollack S M, Cimino E F, Robbins D S, Hoffman P M

出版信息

J Clin Microbiol. 1986 May;23(5):951-3. doi: 10.1128/jcm.23.5.951-953.1986.

DOI:10.1128/jcm.23.5.951-953.1986
PMID:3519665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268757/
Abstract

A solid-phase immunoadsorption procedure (Quantigen T&B cell kit; Bio-Rad Laboratories, Richmond, Calif.) employing monoclonal antibody T101 detected mean percentages of peripheral blood T cells comparable to those obtained by rosetting with sheep erythrocytes, while lower values were obtained with an indirect immunofluorescence procedure (Cytotag T&B cell kit; Hybritech, Inc., San Diego, Calif.) employing the same antibody. Therefore, T101 binding appears to be more easily detected by solid-phase immunoadsorption than by immunofluorescence microscopy.

摘要

一种使用单克隆抗体T101的固相免疫吸附程序（Quantigen T&B细胞试剂盒；伯乐公司，加利福尼亚州里士满）检测到的外周血T细胞平均百分比与用绵羊红细胞进行玫瑰花结试验所获得的结果相当，而使用相同抗体的间接免疫荧光程序（Cytotag T&B细胞试剂盒；Hybritech公司，加利福尼亚州圣地亚哥）得到的值较低。因此，与免疫荧光显微镜相比，固相免疫吸附似乎更容易检测到T101的结合。

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T-cell detection with monoclonal antibody T101 kits.使用单克隆抗体T101试剂盒进行T细胞检测。
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[Immunofluorescence tests versus rosette tests. Detection of receptors for the formation of rosettes with sheep erythrocytes using monoclonal antibodies OKT-11 and OKT-11A].[免疫荧光试验与玫瑰花结试验。使用单克隆抗体OKT-11和OKT-11A检测与绵羊红细胞形成玫瑰花结的受体]
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引用本文的文献

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Monoclonal antibodies for clinical applications. Patents and literature.临床应用的单克隆抗体。专利与文献。
Appl Biochem Biotechnol. 1988 Dec;19(3):271-96. doi: 10.1007/BF02921499.

本文引用的文献

1
Human T cell antigens defined by monoclonal antibodies: the 65,000-dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin.由单克隆抗体定义的人类T细胞抗原：T细胞的65000道尔顿抗原（T65）也存在于带有表面免疫球蛋白的慢性淋巴细胞白血病细胞上。
J Immunol. 1980 Aug;125(2):725-31.
2
Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage.人类胸腺内分化的离散阶段：T细胞系正常胸腺细胞和白血病淋巴母细胞的分析
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588-92. doi: 10.1073/pnas.77.3.1588.
3
Binding of sheep red blood cells to a large population of human lymphocytes.绵羊红细胞与大量人类淋巴细胞的结合。
Nature. 1971 Apr 23;230(5295):531-2. doi: 10.1038/230531a0.
4
The human rosette-forming cell as a marker of a population of thymus-derived cells.人玫瑰花结形成细胞作为胸腺来源细胞群体的标志物。
J Clin Invest. 1972 Oct;51(10):2537-43. doi: 10.1172/JCI107069.
5
Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
6
A methodological study of E-rosette formation using AET-treated sheep red blood cells.一项使用经AET处理的绵羊红细胞进行E花环形成的方法学研究。
J Immunol Methods. 1979 May 10;27(1):61-74. doi: 10.1016/0022-1759(79)90239-4.
7
A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human T cells.一种与功能成熟的人胸腺细胞和所有外周血人T细胞具有选择性反应性的单克隆抗体。
J Immunol. 1979 Sep;123(3):1312-7.

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