Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou 350002, China.
J Agric Food Chem. 2022 Mar 9;70(9):3067-3078. doi: 10.1021/acs.jafc.1c08248. Epub 2022 Feb 24.
Heterosis or hybrid vigor is extensively used in plant breeding. However, the contribution of metabolites to heterosis is still elusive. Here, we systematically identified the non-volatile and volatile metabolites of two hybrids and their parents in . The metabolomics analysis showed prevalent non-additive accumulation in hybrids, among which the non-additive nucleotides, alkaloids, organic acids, and tannins contribute to the positive heterosis of hybrids, including typical inosine, guanosine, adenosine, caffeine, succinic acid, adipic acid, xylonic acid, and gallic acid. The catechins and free amino acids in hybrids showed negative heterosis compared to its maternal cultivar TGY. Furthermore, the significant accumulation of non-additive terpenes combined with the mild heterosis of other types of volatiles contributes to the aroma of tea plant hybrids. The genetics of volatiles from different parents affect the aroma of hybrids processed into oolong tea. The comprehensive heterosis of these non-additive metabolites may play an important role in the formation of desirable breeding traits for hybrids. Our results provide insights into the utilization of heterosis breeding and the regulation of heterosis metabolites in tea plants.
杂种优势或杂种优势在植物育种中被广泛应用。然而，代谢物对杂种优势的贡献仍然难以捉摸。在这里，我们系统地鉴定了两个杂种及其亲本在中的非挥发性和挥发性代谢物。代谢组学分析表明杂种中普遍存在非加性积累，其中非加性核苷酸、生物碱、有机酸和单宁有助于杂种的正杂种优势，包括典型的肌苷、鸟苷、腺苷、咖啡因、琥珀酸、己二酸、戊二酸和没食子酸。与母本品种 TGY 相比，杂种中的儿茶素和游离氨基酸表现出负杂种优势。此外，非加性萜类化合物的显著积累与其他类型挥发性物质的温和杂种优势相结合，有助于茶树杂种的香气形成。不同亲本来源的挥发性物质的遗传差异影响加工成乌龙茶的杂种的香气。这些非加性代谢物的综合杂种优势可能在杂种理想育种特性的形成中发挥重要作用。我们的研究结果为杂种优势利用和茶树杂种优势代谢物的调控提供了新的思路。
