InventicsDx GmbH, Magnusstrasse 11, 12489 Berlin, Germany.
Biological Physics, Institute of Physics and Astronomy, University of Potsdam, Karl-Liebknecht-Strasse 24/25, 14476 Potsdam, Germany.
Biosensors (Basel). 2022 Jan 24;12(2):62. doi: 10.3390/bios12020062.
In this work, the fabrication and characterization of a simple, inexpensive, and effective microfluidic paper analytic device (µPAD) for monitoring DNA samples is reported. The glass microfiber-based chip has been fabricated by a new wax-based transfer-printing technique and an electrode printing process. It is capable of moving DNA effectively in a time-dependent fashion. The nucleic acid sample is not damaged by this process and is accumulated in front of the anode, but not directly on the electrode. Thus, further DNA processing is feasible. The system allows the DNA to be purified by separating it from other components in sample mixtures such as proteins. Furthermore, it is demonstrated that DNA can be moved through several layers of the glass fiber material. This proof of concept will provide the basis for the development of rapid test systems, e.g., for the detection of pathogens in water samples.
本工作报道了一种简单、廉价且有效的用于监测 DNA 样本的微流控纸分析器件（µPAD）的制作和特性。这种基于玻璃微纤维的芯片是通过新型基于蜡的转印技术和电极印刷工艺制作的，能够以时间依赖的方式有效地移动 DNA。该过程不会损坏核酸样品，并将其积累在阳极前，但不会直接在电极上。因此，可以进行进一步的 DNA 处理。该系统允许通过将 DNA 从样品混合物中的其他成分（如蛋白质）中分离出来进行 DNA 纯化。此外，还证明了 DNA 可以通过玻璃纤维材料的多层进行移动。这一概念验证将为快速测试系统的开发提供基础，例如用于检测水样中的病原体。
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