Nguyen Peter Q, Soenksen Luis R, Donghia Nina M, Angenent-Mari Nicolaas M, de Puig Helena, Huang Ally, Lee Rose, Slomovic Shimyn, Galbersanini Tommaso, Lansberry Geoffrey, Sallum Hani M, Zhao Evan M, Niemi James B, Collins James J
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
Nat Biotechnol. 2021 Nov;39(11):1366-1374. doi: 10.1038/s41587-021-00950-3. Epub 2021 Jun 28.
Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of living, engineered bacteria, which has limited their application in wearables. Here we report lightweight, flexible substrates and textiles functionalized with freeze-dried, cell-free synthetic circuits, including CRISPR-based tools, that detect metabolites, chemicals and pathogen nucleic acid signatures. The wearable devices are activated upon rehydration from aqueous exposure events and report the presence of specific molecular targets by colorimetric changes or via an optical fiber network that detects fluorescent and luminescent outputs. The detection limits for nucleic acids rival current laboratory methods such as quantitative PCR. We demonstrate the development of a face mask with a lyophilized CRISPR sensor for wearable, noninvasive detection of SARS-CoV-2 at room temperature within 90 min, requiring no user intervention other than the press of a button.
将合成生物学集成到可穿戴设备中，可以扩大对生理状态、疾病状态以及病原体或毒素暴露情况进行无创监测的机会。然而，合成电路的运行通常需要有经过工程改造的活细菌存在，这限制了它们在可穿戴设备中的应用。在此，我们报告了用冻干的无细胞合成电路功能化的轻质、柔性基板和纺织品，这些合成电路包括基于CRISPR的工具，可检测代谢物、化学物质和病原体核酸特征。可穿戴设备在因接触水而重新水化时被激活，并通过比色变化或通过检测荧光和发光输出的光纤网络报告特定分子靶标的存在。核酸的检测限可与当前的实验室方法（如定量PCR）相媲美。我们展示了一种带有冻干CRISPR传感器的口罩的开发，用于在室温下90分钟内对SARS-CoV-2进行可穿戴、无创检测，除了按下一个按钮外无需用户干预。
