Shved Natalia, Egorova Anna, Osinovskaya Natalia, Kiselev Anton
Department of Genomic Medicine, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, MendeleevskayaLine 3, 199034 Saint-Petersburg, Russia.
Methods Protoc. 2022 Feb 6;5(1):16. doi: 10.3390/mps5010016.
Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in and genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested.
细胞技术是生物医学最具前景的领域之一，它基于分离各种类型的细胞，随后进行培养和利用，或利用其代谢产物用于医学目的。如今，很大一部分生物医学研究是在体外进行的。另一方面，器官型培养可作为一种强大的模型系统，在不同的生物医学应用中补充细胞培养和体内研究。子宫平滑肌瘤（UL）是一种非常常见的良性肿瘤，常导致许多生殖并发症。在此，我们描述了一种快速可靠的子宫平滑肌瘤原代细胞分离和培养方法，以及子宫平滑肌瘤器官型模型的建立。我们建议在第一代实验工作中使用子宫平滑肌瘤原代细胞，以防止在 和 基因中驱动突变的丢失。本文提出了体外子宫平滑肌瘤二维和三维模型生长和维持的新优化条件。
