Gali P, Wolfrom C, Gautier M, Hartmann L
Liver. 1986 Feb;6(1):53-9. doi: 10.1111/j.1600-0676.1986.tb00268.x.
In the basal state, the presence of L pyruvate kinase (LPK) was constantly observed in primary human liver cell cultures initiated from explants, when cells were examined by immunofluorescence and double labelling. After short-term insulin incubation, M pyruvate kinase (MPK) appeared. Therefore, both LPK and MPK were located in the same cells. We previously obtained the same results in isolated rat hepatocytes in which we demonstrated that short-term regulation of M2PK by insulin was a function of dose and/or incubation time. The present work established that similar conditions govern the regulation of this isozyme in vitro in human hepatocytes.
在基础状态下，当通过免疫荧光和双重标记检查外植体来源的原代人肝细胞培养物时，始终能观察到L型丙酮酸激酶（LPK）的存在。短期胰岛素孵育后，M型丙酮酸激酶（MPK）出现。因此，LPK和MPK位于同一细胞中。我们之前在分离的大鼠肝细胞中也得到了相同的结果，在该实验中我们证明胰岛素对M2PK的短期调节是剂量和/或孵育时间的函数。目前的研究确定，在体外人肝细胞中，类似的条件控制着这种同工酶的调节。
