某些已知遗传毒性剂经猴（食蟹猴）分离肝细胞活化后对鼠伤寒沙门氏菌的致突变性。与分离的人肝细胞的比较。

Mutagenicity towards Salmonella typhimurium of some known genotoxic agents, activated by isolated hepatocytes of monkey (Macaca fascicularis). Comparison with isolated human hepatocytes.

作者信息

Neis J M, Roelofs H M, van Gemert P J, Henderson P T

出版信息

Mutat Res. 1986 Jun;164(3):139-43. doi: 10.1016/0165-1161(86)90005-1.

PMID:3520301

Abstract

This paper describes some striking differences between isolated human and monkey hepatocytes in their capacity to activate some known genotoxic agents into products mutagenic towards Salmonella typhimurium. Isolated monkey hepatocytes, in contrast to human hepatocytes, appeared to activate benzidine (BZ), N-acetylbenzidine (MABZ), N,N'-diacetylbenzidine (DABZ), 2-aminofluorene (2-AF) and 2-acetylaminofluorene (2-AAF) poorly. With monkey hepatocytes BZ was slightly more mutagenic than DABZ, whereas with human hepatocytes DABZ was more active than BZ. N-Nitrosodimethylamine (DMN) and N-nitrosodiethylamine (DEN) were also found to be poorly mutagenic when activated by monkey hepatocytes, unlike the human hepatocytes. However, the polycyclic arylhydrocarbons benzo[a]pyrene (B[a]P) and 7,12-dimethylbenzanthracene (7,12-DMBA) were highly active in the presence of monkey hepatocytes, unlike the human hepatocytes. A metabolic study showed that monkey liver preparations seem to possess a higher monooxygenase activity towards B[a]P than human liver preparations.

摘要

本文描述了分离出的人肝细胞和猴肝细胞在激活某些已知的遗传毒性剂使其对鼠伤寒沙门氏菌产生致突变产物的能力方面存在的一些显著差异。与人类肝细胞相比，分离出的猴肝细胞似乎对联苯胺（BZ）、N - 乙酰联苯胺（MABZ）、N，N'-二乙酰联苯胺（DABZ）、2 - 氨基芴（2 - AF）和2 - 乙酰氨基芴（2 - AAF）的激活能力较差。对于猴肝细胞，BZ的致突变性略高于DABZ，而对于人类肝细胞，DABZ比BZ更具活性。与人类肝细胞不同，当由猴肝细胞激活时，N - 亚硝基二甲胺（DMN）和N - 亚硝基二乙胺（DEN）的致突变性也很低。然而，与人类肝细胞不同，多环芳烃苯并[a]芘（B[a]P）和7,12 - 二甲基苯并蒽（7,12 - DMBA）在猴肝细胞存在时具有高活性。一项代谢研究表明，猴肝制剂对B[a]P的单加氧酶活性似乎高于人肝制剂。