Wakai S, Meiselman S E, Brightman M W
Neurosurgery. 1986 May;18(5):548-54. doi: 10.1227/00006123-198605000-00006.
Nuchal muscle autografts of two different sizes were transplanted into rat brain parenchyma (intraparenchymal, 1.5 X 1.5 X 1 mm) and onto the surface of the brain stem (intraventricular, 2 X 2 X 1 and 1.5 X 1.5 X 1 mm). The vasculature of the transplants retained its permeability to proteins. Exogenous, intravenously injected horseradish peroxidase (HRP) and endogenous immunoglobulins (IgG) crossed the vessels of the grafts to enter the surrounding brain tissue 1 and 3 months after transplantation. HRP infiltrated about 0.46 to 4.6 mm into the extracellular spaces around the grafts 60 minutes after its intravenous injection. The penetration of HRP depended on the size and age of the graft. Infiltration was greater in 1-month-old rats with slightly larger intraventricular grafts than in those with smaller grafts. There was a tendency for the penetration of HRP to be greater from 1-month-old grafts than from 3-month-old grafts, but the difference was not statistically significant, except for the horizontal vector of spread in the intraparenchymal group. Although endogenous IgG infiltrated the surrounding brain tissue, its penetration was very limited in comparison with that of HRP. The results suggest that muscle grafts could be used as a readily available and accessible means of circumventing the blood-brain barrier selectively and focally.
将两种不同大小的颈部肌肉自体移植物移植到大鼠脑实质内（脑实质内，1.5×1.5×1毫米）和脑干表面（脑室内，2×2×1毫米和1.5×1.5×1毫米）。移植物的血管系统保持其对蛋白质的通透性。在移植后1个月和3个月，静脉注射的外源性辣根过氧化物酶（HRP）和内源性免疫球蛋白（IgG）穿过移植物的血管进入周围脑组织。静脉注射HRP 60分钟后，HRP渗入移植物周围细胞外间隙约0.46至4.6毫米。HRP的渗透取决于移植物的大小和年龄。在1月龄大鼠中，脑室内稍大的移植物比小移植物的HRP渗入更多。1月龄移植物的HRP渗透有比3月龄移植物更大的趋势，但除了脑实质内组的水平扩散向量外，差异无统计学意义。尽管内源性IgG渗入周围脑组织，但其渗透与HRP相比非常有限。结果表明，肌肉移植物可作为一种容易获得且可利用的手段，选择性地、局部地绕过血脑屏障。
